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Antonie van Leeuwenhoek

, Volume 111, Issue 7, pp 1047–1054 | Cite as

Streptomyces flavalbus sp. nov., an actinobacterium isolated from rhizosphere of maize (Zea mays L.)

  • Tingting Cao
  • Yibo Shen
  • Junwei Zhao
  • Chongxi Liu
  • Xueli Zhao
  • Liying Jin
  • Yanjie Li
  • Xiangjing Wang
  • Wensheng Xiang
Original Paper

Abstract

A novel actinobacterium, designated strain NEAU-QY24T, was isolated from the rhizosphere of corn (Zea mays L.). A polyphasic approach was employed to determine the taxonomic status of strain NEAU-QY24T. The isolate was found to have chemical and morphological properties of the genus Streptomyces, with high 16S rRNA gene sequence similarity to Streptomyces lanatus JCM 4332T (98.3%) and clustered phylogenetically with Streptomyces lannensis JCM 16578T (98.2%). The cell wall was found to contain meso-diaminopimelic acid and the whole cell sugars were identified as glucose and ribose. The predominant menaquinones were identified as MK-9(H6), MK-9(H4) and MK-9(H8). The phospholipid profile was found to consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were identified as iso-C16:0, C16:0, anteiso-C17:0 and C15:0. A combination of DNA–DNA hybridization experiments and phenotypic tests were carried out between strain NEAU-QY24T and its closely related strains, which clarified their relatedness and demonstrated that strain NEAU-QY24T could be distinguished from these strains. These data indicate that the isolate should be recognised as a new species of the genus Streptomyces, for which the name Streptomyces flavalbus sp. nov. is proposed. The type strain is NEAU-QY24T (= CGMCC 4.7400T = DSM 104539T).

Keywords

Streptomyces flavalbus sp. nov. Polyphasic approach 16S rRNA gene Rhizosphere of corn 

Notes

Acknowledgements

This work was supported in part by grants from the National Natural Science Foundation of China (No. 31471832), Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants and/or animals performed by any of the authors. The formal consent is not required in this study.

Supplementary material

10482_2017_1004_MOESM1_ESM.doc (709 kb)
Supplementary material 1 (DOC 709 kb)

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Tingting Cao
    • 1
    • 2
  • Yibo Shen
    • 2
  • Junwei Zhao
    • 1
    • 2
  • Chongxi Liu
    • 2
  • Xueli Zhao
    • 2
  • Liying Jin
    • 2
  • Yanjie Li
    • 3
  • Xiangjing Wang
    • 2
  • Wensheng Xiang
    • 1
    • 2
  1. 1.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China
  2. 2.Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education CommitteeNortheast Agricultural UniversityHarbinPeople’s Republic of China
  3. 3.Heihe Branch of Heilongjiang Academy of Agricultural SciencesHeihePeople’s Republic of China

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