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Streptomyces flavalbus sp. nov., an actinobacterium isolated from rhizosphere of maize (Zea mays L.)

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Abstract

A novel actinobacterium, designated strain NEAU-QY24T, was isolated from the rhizosphere of corn (Zea mays L.). A polyphasic approach was employed to determine the taxonomic status of strain NEAU-QY24T. The isolate was found to have chemical and morphological properties of the genus Streptomyces, with high 16S rRNA gene sequence similarity to Streptomyces lanatus JCM 4332T (98.3%) and clustered phylogenetically with Streptomyces lannensis JCM 16578T (98.2%). The cell wall was found to contain meso-diaminopimelic acid and the whole cell sugars were identified as glucose and ribose. The predominant menaquinones were identified as MK-9(H6), MK-9(H4) and MK-9(H8). The phospholipid profile was found to consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were identified as iso-C16:0, C16:0, anteiso-C17:0 and C15:0. A combination of DNA–DNA hybridization experiments and phenotypic tests were carried out between strain NEAU-QY24T and its closely related strains, which clarified their relatedness and demonstrated that strain NEAU-QY24T could be distinguished from these strains. These data indicate that the isolate should be recognised as a new species of the genus Streptomyces, for which the name Streptomyces flavalbus sp. nov. is proposed. The type strain is NEAU-QY24T (= CGMCC 4.7400T = DSM 104539T).

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Acknowledgements

This work was supported in part by grants from the National Natural Science Foundation of China (No. 31471832), Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP).

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Authors and Affiliations

  1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

    Tingting Cao, Junwei Zhao & Wensheng Xiang

  2. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

    Tingting Cao, Yibo Shen, Junwei Zhao, Chongxi Liu, Xueli Zhao, Liying Jin, Xiangjing Wang & Wensheng Xiang

  3. Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe, Heilongjiang, People’s Republic of China

    Yanjie Li

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  1. Tingting Cao
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Cao, T., Shen, Y., Zhao, J. et al. Streptomyces flavalbus sp. nov., an actinobacterium isolated from rhizosphere of maize (Zea mays L.). Antonie van Leeuwenhoek 111, 1047–1054 (2018). https://doi.org/10.1007/s10482-017-1004-6

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