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Rothia marina sp. nov., isolated from an intertidal sediment of the South China Sea

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Abstract

A novel non-sporulating, non-motile, catalase-positive, oxidase-negative, facultatively anaerobic, Gram-positive coccus, designated strain JSM 078151T, was isolated from an intertidal sediment sample collected from Naozhou Island in the South China Sea, China. Growth was found to occur in the presence of 0–15 % (w/v) NaCl (optimum 0.5–3 % (w/v) NaCl), at pH 6.5–10.5 (optimum pH 7.0–8.0) and at 5–35 °C (optimum 25–30 °C). The peptidoglycan type was determined to be A3a, containing lysine, glutamic acid and alanine. The major cellular fatty acid identified was anteiso-C15:0 and the predominant menaquinones are MK-7 and MK-8. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylglycerol, glycolipid and one unidentified phospholipid. The genomic DNA G+C content of strain JSM 078151T was determined to be 55.2 mol%. A phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 078151T should be assigned to the genus Rothia, and was most closely related to Rothia nasimurium CCUG 35957T (98.3 % sequence similarity), followed by Rothia amarae J18T (97.5 %) and Rothia terrae L-143T (97.3 %). A combination of phylogenetic analysis, DNA–DNA relatedness values, phenotypic characteristics and chemotaxonomic data supports the suggestion that strain JSM 078151T represents a novel species of the genus Rothia, for which the name Rothia marina sp. nov. is proposed. The type strain is JSM 078151T (= DSM 21080T = KCTC 19432T).

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Acknowledgments

This work was supported by Grants from the National Basic Research Program of China (2010CB833800), National Natural Science Foundation of China (NSFC) (30970007), Construct Program of the Key Discipline in Hunan Province (JSU071312Z01), Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province (201208Z01), the Education Department of Hunan Province (12K108) and Jiugui Liquor Co. Ltd. (JGKJ 2012-JD03).

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Authors and Affiliations

  1. College of Biology and Environmental Sciences, Jishou University, Jishou, 416000, People’s Republic of China

    Zhu-Xiang Liu, He Liu & Yi-Guang Chen

  2. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Kunming, 650091, People’s Republic of China

    Ling-Ling Yang, Shu-Kun Tang & Wen-Jun Li

  3. State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Ying Huang

  4. Jiugui Liquor Co. Ltd., Jishou, 416000, People’s Republic of China

    Hu Zhao

  5. College of Life Sciences, Hunan Normal University, Changsha, 410081, People’s Republic of China

    Zhu-Xiang Liu

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  1. Zhu-Xiang Liu
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Liu, ZX., Yang, LL., Huang, Y. et al. Rothia marina sp. nov., isolated from an intertidal sediment of the South China Sea. Antonie van Leeuwenhoek 104, 331–337 (2013). https://doi.org/10.1007/s10482-013-9955-8

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