Verrucosispora maris sp. nov., a novel deep-sea actinomycete isolated from a marine sediment which produces abyssomicins

Abstract

Verrucosispora isolate AB-18-032T, the abyssomicin- and proximicin-producing actinomycete, has chemotaxonomic and morphological properties consistent with its classification in the genus Verrucosispora. The organism formed a distinct phyletic line in the Verrucosispora 16S rRNA gene tree sharing similarities of 99.7%, 98.7% and 98.9% with Verrucosispora gifhornensis DSM 44337T, Verrucosispora lutea YIM 013T and Verrucosispora sediminis MS 426T, respectively. It was readily distinguished from the two latter species using a range of phenotypic features and from V. gifhornensis DSM 44337T, its nearest phylogenetic neighbor, by a DNA G+C content of 65.5 mol% obtained by thermal denaturation and fluorometry and DNA:DNA relatedness values of 64.0% and 65.0% using renaturation and fluorometric methods, respectively. It is apparent from the combined genotypic and phenotypic data that strain AB-18-032T should be classified in the genus Verrucosispora as a new species. The name Verrucosispora maris sp. nov. is proposed for this taxon with isolate AB-18-032T (= DSM 45365T = NRRL B-24793T) as the type strain.

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Acknowledgments

ATB and MG were supported by the UK Natural Environmental Research Council (grants NER/T/S/2000/00614 and NER/T/S/2000/00616), JEMS by the Biological and Biotechnological Research Council (BBSRC grant BB/EO17053/1) and TDZ by a Conselho Nacional de DesenvolvImento Cientifico e Tecnológico Fellowship to study in the UK (grant 201066/2009-2). ATB thanks The Leverhulme Trust for an Emeritus Fellowship. The authors are indebted to Professor Koki Horikoshi (JAMSTEC) for the sediment sample.

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Correspondence to Michael Goodfellow.

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The Genbank accession number for the 16S rRNA gene sequence of Verrucosispora maris AB-18-032T is AY528866.

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Goodfellow, M., Stach, J.E.M., Brown, R. et al. Verrucosispora maris sp. nov., a novel deep-sea actinomycete isolated from a marine sediment which produces abyssomicins. Antonie van Leeuwenhoek 101, 185–193 (2012). https://doi.org/10.1007/s10482-011-9651-5

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Keywords

  • Verrucosispora maris
  • Polyphasic taxonomy
  • Marine sediment
  • Actinomycete