Ornithinibacillus scapharcae sp. nov., isolated from a dead ark clam

Abstract

A novel Gram-positive, aerobic, motile, hemolytic, endospore-forming and rod-shaped bacterium TW25T was isolated from a dead ark clam during a mass mortality event on the South coast of Korea. The strain grew optimally at 30°C, at pH 8–9, and with 1% (w/v) NaCl. The 16S rRNA gene sequence analysis indicated that strain TW25T was associated with the genus Ornithinibacillus and that it was most closely related to the type strain of Ornithinibacillus californiensis (98.5% similarity). The dominant cellular fatty acids were iso-C15:0, anteiso-C15:0 and C16:0. The peptidoglycan amino acid type was A4β, containing l-ornithine and d-aspartic acid. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified phospholipids, two unidentified aminolipids and two unidentified lipids. The major respiratory quinone was menaquinone-7 (MK-7). The G + C content of genomic DNA was 36.7 mol%. DNA–DNA hybridization experiments with related strains revealed lower than 11 ± 3% relatedness. Based on this polyphasic taxonomic study, strain TW25T represents a novel species in the genus Ornithinibacillus, for which the name Ornithinibacillus scapharcae sp. nov. is proposed. The type strain is TW25T (=KACC 15116T = JCM 17314T).

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Acknowledgments

We thank Dr. J. P. Euzéby (École Nationale Vétérinaire, France) for etymological advice. This work was supported by 21C Frontier Microbial Genomics and Application Center Program, and the grant from the National Fisheries Research and Development Institute (NFRDI), Republic of Korea. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain TW25T is HQ171440.

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Correspondence to Jin-Woo Bae.

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Na-Ri Shin and Tae Woong Whon contributed equally to this work.

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Shin, NR., Whon, T.W., Kim, MS. et al. Ornithinibacillus scapharcae sp. nov., isolated from a dead ark clam. Antonie van Leeuwenhoek 101, 147–154 (2012). https://doi.org/10.1007/s10482-011-9645-3

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Keywords

  • Ornithinibacillus scapharacae
  • Polyphasic taxonomy
  • Ark clam
  • A4β type peptidoglycan