A straw-yellow pigmented bacterium, strain IITR-21T was isolated from a pesticide contaminated site and characterized by using a polyphasic taxonomic approach. The organism had morphological and chemotaxonomic properties consistent with its classification in the genus Nitratireductor. Phylogenetic analysis of the 16S rRNA gene sequence showed that the strain IITR-21T belongs to the genus Nitratireductor and was moderately related to Nitratireductor indicus C115T (97.7%) and Nitratireductor pacificus pht-3BT (97.4%), whereas sequence similarity value with the other species including the type species of the genus Nitratireductor, Nitratireductor aquibiodomus showed less than 97.0% similarity. However, the DNA–DNA relatedness values between strain IITR-21T and the moderately related taxa N. indicus (59.1%) and N. pacificus (40.4%) were well below the 70% threshold value recommended for the delineation of bacterial species. The G+C content of the DNA was 62.4 mol%. Based on physiological, biochemical tests and genotypic differences between the strain IITR-21T and the other two validly published species of the genus Nitratireductor, it is proposed that the strain be classified as a new species of Nitratireductor as Nitratireductor lucknowense sp. nov. The type strain is IITR-21T (=MTCC 8354T = DSM 24322T).
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We thank Mr. Malkit Singh for his excellent technical assistance. This work was supported by CSIR (Net Work Project NWP006). Government of India. The IMTECH communication number is 010/2011.
Natesan Manickam and Siddhika Pareek have contributed equally to this study.
The GenBank accession number for the 16S rDNA sequence of strain IITR-21T is HQ658355.
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Manickam, N., Pareek, S., Kaur, I. et al. Nitratireductor lucknowense sp. nov., a novel bacterium isolated from a pesticide contaminated soil. Antonie van Leeuwenhoek 101, 125–131 (2012). https://doi.org/10.1007/s10482-011-9623-9
- DNA–DNA hybridization
- Fatty acid methyl ester