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Bacillus hunanensis sp. nov., a slightly halophilic bacterium isolated from non-saline forest soil

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Abstract

A novel Gram-stain-positive, slightly halophilic, catalase- and oxidase-positive, endospore-forming, motile, aerobic, rod-shaped bacterium, designated strain JSM 081003T, was isolated from non-saline forest soil in Hunan Province, China. Growth occurred with 0.5–15% (w/v) NaCl (optimum 2–4%) at pH 6.5–10.5 (optimum pH 7.5–8.5) and at 5–40°C (optimum 30°C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were iso-C15:0, anteiso-C15:0 and iso-C14:0. Strain JSM 081003T contained MK-7 as the predominant respiratory quinone, and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. The genomic DNA G + C content of strain JSM 081003T was 40.9 mol%. A phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 081003T should be assigned to the genus Bacillus, and was related most closely to the type strains of Bacillus lehensis (sequence similarity 99.6%), Bacillus oshimensis (99.4%) and Bacillus patagoniensis (96.6%); lower than 96.0% sequence similarity was observed with other Bacillus species. The combination of phylogenetic analysis, DNA–DNA relatedness values, phenotypic characteristics and chemotaxonomic data supports the view that strain JSM 081003T represents a new species of the genus Bacillus, for which the name Bacillus hunanensis sp. nov. is proposed. The type strain is JSM 081003T (= DSM 23008T = KCTC 13711T).

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Acknowledgments

This work was supported by grants from the National Basic Research Program of China (2010CB833800), National Natural Science Foundation of China (NSFC) (30970007, 30970008), Key Laboratory of Ecotourism’s Application Technology of Hunan Province (SL2010Z01), Key Laboratory of Hunan Forest Products and Chemical Industry Engineering (JDZ200907) and State-level Public Welfare Scientific Research Institutes for Basic R&D Special Fund (IMBF200915). We are grateful to Mr Zhu-Xiang Liu and Mr Ke Huang for their excellent technical assistance.

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Authors and Affiliations

  1. Key Laboratory of Ecotourism’s Application Technology of Hunan Province, College of Biology and Environmental Sciences, Jishou University, 416000, Jishou, People’s Republic of China

    Yi-Guang Chen, Qi-Hui Chen, Jian-Ben Liu & Jian-Wu He

  2. Key Laboratory of Ecotourism’s Application Technology of Hunan Province, College of Chemistry and Chemical Engineering, Jishou University, 416000, Jishou, People’s Republic of China

    Yi-Guang Chen, Qi-Hui Chen, Jian-Ben Liu & Jian-Wu He

  3. The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, 650091, Kunming, People’s Republic of China

    Shu-Kun Tang & Wen-Jun Li

  4. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, 100050, Beijing, People’s Republic of China

    Yu-Qin Zhang

  5. Department of Food Engineering, Jiangsu Food Science College, 223003, Huaian, People’s Republic of China

    Di-Fei Hao

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  1. Yi-Guang Chen
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Correspondence to Yi-Guang Chen.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 081003T is HM054473.

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Chen, YG., Hao, DF., Chen, QH. et al. Bacillus hunanensis sp. nov., a slightly halophilic bacterium isolated from non-saline forest soil. Antonie van Leeuwenhoek 99, 481–488 (2011). https://doi.org/10.1007/s10482-010-9512-7

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