Agroforestry Systems

, Volume 92, Issue 1, pp 91–101 | Cite as

Diversity and abundance of arbuscular mycorrhizal fungi under different plant and soil properties in Sidama, southern Ethiopia

Article

Abstract

In Sidama, agroforestry represents land-use systems with deliberate management of multipurpose trees and shrubs that grow in intimate association with annual and perennial agricultural crops and/or livestock. The interaction of microbiota with the trees, shrubs and crops may make the system fertile, productive and sustainable. One of the beneficial microbiota which has symbiotic association with most of the plants in agroforestry is arbuscular mycorrhizal fungi (AMF). In November and December of 2012, root and rhizosphere soil samples of 21 plant species from nine peasant associations (PAs) (villages within districts where 300–500 families live) were collected from the agroforestry practices in Sidama of Southern Ethiopia for the determination of diversity and abundance of AMF under selected soil parameters and plant species density. Findings on the diversity of AMF based on soil properties showed that at moderate to low P and N concentrations the rate of AMF root colonization and spore density was high in comparison with the rhizosphere soils with the highest P and N concentration. The highest percentage of total AMF colonization was recorded for shade trees Millettia ferruginea (84 %) and Erythrina brucei (80 %) followed by intercropped perennial crops Ensete ventricosum (86 %), Catha edulis (85 %) and Coffea arabica (80 %) and the lowest percentage AMF colonization was recorded for Rhamnus prinoides (53 %) and Colocasia esculenta (52 %). Though found in almost all homegarden agroforestry practices and with broad coverage in Sidama agroforestry, some crops and vegetables such Brassica integrifolia and Cucurbita pepo, grown intercropped were found to be non-mycorrhizal as none of the AMF structures were recorded. The highest number of AM spore population was recorded in rhizosphere soils of Croton macrostachyus (1066 ± 19.33) and Catha edulis (1054 ± 53.12) and the lowest spore density was recorded for Dioscorea alata (100.00 ± 2.89) spore per 100 g of dry soil. The percentage fungal colonization in any individual plant species and spore population in the rhizosphere soils of that species did not correlate to each other and percentage AM root colonization and spore density of all plants in the agroforestry of Sidama were found significantly different at P < 0.05 level.

Keywords

AMF Rhizosphere Parameters Colonization Density 

Notes

Acknowledgments

We would like to acknowledge Hawassa College of Teacher Education for financial and logistic supports; Department of Microbiology, Cellular and Molecular Biology, Addis Ababa University and College of agriculture, Hawassa University for their support with chemicals and laboratory equipments.

References

  1. Allsopp N, Stock WF (1992) Density dependent interactions between VA mycorrhizal fungi and even-aged seedlings of two perennial Fabaceae species. Oecologia 91:281–287. doi: 10.1007/BF00317797 CrossRefPubMedGoogle Scholar
  2. Belay Z, Asefa F, Mauritz V (2014) Diversity and abundance of arbuscular mycorrhizal fungi associated with acacia trees from different land use systems in Ethiopia. Afr J Microb Res 7(48):550Google Scholar
  3. Bierman B, Linderman RG (1983) Use of vesicular-arbuscular mycorrhizal roots, intraradicle vesicles and extraradicle vesicles asinoculum. New Phyto 95:97–105CrossRefGoogle Scholar
  4. Black C, Evans AD, White JL, Ensminger LE, Clark FE (1965) Methods of soil analysis part 1. American Society of Agronomy, Madison, Wis, USA, Physical and Mineralogical Properties Including Statistics of Measurement and SamplingGoogle Scholar
  5. Blaszkowski J (1989) The occurrence of the Endogonaceae in Poland. Agric Ecosy Environ 29:45–50CrossRefGoogle Scholar
  6. Bolan NS, Robson AD, Barrow NJ (1987) Effects of vesicular–arbuscular mycorrhiza on theavailability of iron phosphates to plants. Plant Soil 99:401–410CrossRefGoogle Scholar
  7. Bremner JM, Mulvaney SC (1982) Nitrogen-total. In: Page AL (ed) Methods of soil analysis, vol 2. American Society of Agronomy, Madison, pp 595–624Google Scholar
  8. da Silva CS, Menezes RSC, de Sampaio EVSB, Oehl F, Maia LC (2013) Arbuscular mycorrhizal fungi within agroforestry and traditional land use systems in semi-arid northeast Brazil. Acta Scient Agron 35:307–314Google Scholar
  9. Dalpe Y, Plenchette C, Gueye M (2000) Glomales species associated with surface and deep rhizosphere of Faidherbia albida in Senegal. Mycorrhiza 10:125–129CrossRefGoogle Scholar
  10. Demir S, Kaya I, Şavur OB, Özkan OU (2008) Determination of Arbuscular-Mycorrhizal fungus (AMF) from the plants belonging to Gramineae Family in Van Province. YYU J Agric Sci 18(2):103–111Google Scholar
  11. Diaz G, Honrubia M (1994) A mycorrhizal survey of plants growing on mine wastes in southern Spain. Arid Soil Res Rehabil 8:59–68Google Scholar
  12. Eissenstat DM, Newman EI (1990) Seedling establishment near large plants: effects of vesicular-arbuscular mycorrhizas on the intensity of plant competition. Funct Ecol 4:95–99CrossRefGoogle Scholar
  13. Emiru Birhanea WK, Thomas JS Frank, Frans B (2010) Arbuscular mycorrhizal associations in Boswellia papyrifera (frankincense-tree) dominated dry deciduous woodlands of northern Ethiopia. For Ecol Manag 260:2160–2169CrossRefGoogle Scholar
  14. Fisher RA, Yates F (1970) Statistical tables for biological, agriculture and medical research, 6th edn. Hafner, DavienGoogle Scholar
  15. Gerdemann JW, Nicolson TH (1963) Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting. Trans Br Mycol Soc 46:235–244CrossRefGoogle Scholar
  16. Gerdemann JW, Trappe MJ (1974) The Endogonaceae in the Pacific Northwest. Mycologia Memoir, no. 5. The New York Botanical Garden, New YorkGoogle Scholar
  17. Giovannetti M, Nicolson HT (1983) Vesicular–arbuscular mycorrhizas in Italian sand dunes. Trans Br Mycol Soc 80:552–555CrossRefGoogle Scholar
  18. Jasper DA (1992) Management of mycorrhizas in revegetation. In: Robson AD, Abbott LK, Malajczuk N (eds) Proceedings of international symposium on management of mycorrhizas in agriculture, horticulture and forestryGoogle Scholar
  19. Jefwa JM, Sinclair R, Maghembe JA (2006) Diversity of glomale mycorrhizal fungi in maize/sesbania intercrops and maize monocrop systems in Southern Malawi. Agrofor Syst 67:107–114CrossRefGoogle Scholar
  20. Jones BJJ (2001) Laboratory guide for conducting soil tests and plant analysis. CRC Press, DallasGoogle Scholar
  21. Koide RT, Dickie IA (2002) Effects of mycorrhizal fungi on plants populations. Plant Soil 244:307–317. doi: 10.1023/A:1020204004844 CrossRefGoogle Scholar
  22. Li XL, Marschnerm H, George E (1991) Extension of the phosphorus depletion zone in VA mycorrhizal white clover in a calcareous soil. Plant Soil 135:41–48CrossRefGoogle Scholar
  23. Louis L, Lim G (1987) Spore density and root colonization of vesicular–arbuscular mycorrhizas in tropical soil. Trans Br Mycol Soc 88:207–212CrossRefGoogle Scholar
  24. Mahmud RM, Mridha UA, Osman TK, Xu LH, Umemura H (1999) Relationship between edaphic factors and arbuscular mycorrhizal fungi in soils of rubber plantation. The 207th Annual Meeting of Japanese Society of Crop Science, 2–3 April, 1999, Tokyo. Jap J Crop Sci 68 (Extra 1):244–245Google Scholar
  25. Mehrotra VS (1998) Arbuscular mycorrhizal association of plants colonizing coal mine spoils in India. J Agric Sci Camb 130:123–133Google Scholar
  26. Millat-e-Mustafa M (1997) Tropical homegardens: an overview. In: Alam MK, Ahmed FU, Ruhul Amin AM (eds) Agroforestry: Bangladesh perspective. BARC, Trombay, pp 18–33Google Scholar
  27. Olsen SR, Dean LA (1965) Phosphorous. In: Black CA (ed) Methods of soil analysis part 2: chemical and microbiological properties, vol 9. American Society of Agronomy, Madison, pp 1035–1049Google Scholar
  28. Ortaş İ (2002) Do Plants depend on mycorrhizae in terms of nutrient requirement? In: International conference on sustainable land use and management. Çanakkale.Google Scholar
  29. Phillips JM, Hayman DS (1970) Improved procedures for clearing roots and staining parasitic and vesicular–arbuscular mycorrhizal fungi for rapid assessment of infection. Trans Br Mycol Soc 55:158–161CrossRefGoogle Scholar
  30. Power ME, Mills LS (1995) The keystone cops meet in Hilo. Tree 10:182–184PubMedGoogle Scholar
  31. Raintree JB (1997) Agroforestry concepts. In: Alam MK, Ahmed FU, Ruhul Amin AM (eds) Agroforestry: Bangladesh perspective. BARC, Trombay, pp 1–17Google Scholar
  32. Rhodes LH (1980) The use of mycorrhizae in crop production systems. Outlook Agric 10(6):275–281CrossRefGoogle Scholar
  33. Roy I (1997) Cropland agroforestry: the experience of the village and farm forestry programme. In: Alam MK, Ahmed FU, Ruhul Amin AM (eds) Agroforestry: Bangladesh perspective. BARC, Trombay, pp 98–111Google Scholar
  34. Schenck NC, Pérez Y (1990) Manual for the identification of VA mycorrhizal fungi. Synergistic-Publications, GainesvilleGoogle Scholar
  35. Schnitzer M (1982) Total carbon, organic matter and carbon. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis—part 2. Agronomy monograph, vol 9, 2nd edn. American Society of Agronomy, Madison, pp 539–577Google Scholar
  36. Sieverding E (1991) Vesicular-arbuscular mycorrhiza management in tropical agro systems. German Technical Cooperation (GTZ) Eschborn. ISBN 3 88085 462 9Google Scholar
  37. Smith SE, Read DJ (1997) Mycorrhizal symbiosis, 2nd edn. Academic Press, LondonGoogle Scholar
  38. Snoeck D, Abolo D, Jagoret P (2010) Temporal changes in VAM fungi in the cocoa agroforestry systems of central Cameroon. Agrofor Syst 78:323–328CrossRefGoogle Scholar
  39. Talukdar NC, Germida JJ (1993) Occurrence and isolation of vesicular-arbuscular mycorrhizae in cropped field soils of Saskatchewan. Can J Microbial 39:576–586CrossRefGoogle Scholar
  40. Verbruggen E, Toby Kiers E (2010) Evolutionary ecology of mycorrhizal functional diversity in agricultural systems. Evol Appl 3:547–560CrossRefPubMedPubMedCentralGoogle Scholar
  41. Wang FY, Liu RJ, Lin XG (2004) Arbuscular mycorrhizal status of wild plants in salin-alkaline soils of the yellow river delta. Mycorrhiza 14:133–137CrossRefPubMedGoogle Scholar
  42. Zebene A (2003) Tree species diversity, top soil conditions and Arbuscular mycorrhizal Association in the Sidama traditional agroforestry land use, southern Ethiopia. Doctorial Thesis, Department of Forest Management and Products, SLU, Acta Universitatis Sueciae, SilverstriaGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Department of Natural Resources Management and Environmental SciencesHaramaya UniversityHaramayaEthiopia
  2. 2.Department of Microbial, Cellular and Molecular Biology, College of Natural SciencesAddis Ababa UniversityAddis AbabaEthiopia
  3. 3.Wondo Genet College of Forestry and Natural ResourcesHawassa UniversityHawassaEthiopia

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