Examination of callus micro-grafts in Uapaca kirkiana Müell Arg. was carried out with the objective of determining early signs of graft compatibility. Leaves from U. kirkiana, U. nitida and Jatropha curcas trees were used for callus induction. Two pieces of callus were co-cultured on Murashige and Skoog (MS) medium with different supplements. Co-cultured calli were embedded in paraffin wax and dissected. The specimens were stained in safranin and fast green before viewing under a light microscope. Results showed that MS medium with 0.1 mg l−1 thidiazuron (TDZ) and 0.5 mg l−1 naphthaleneacetic acid (NAA) or 1.0 mg l−1 dichlorophenoxyacetic acid (2,4-D) and 0.5 mg l−1 NAA was effective for callus induction. There were no necrotic layers at the unions within U. kirkiana clones and provenances, but a differential growth (irregularity) between U. kirkiana and U. nitida co-cultured calli. Phenol deposits were observed at the union interfaces of U. kirkiana combinations and were high on calli derived from mature trees. Phenol deposits were absent at the union of J. curcas heterografts. Necrotic layers developed at the unions of U. kirkiana and J. curcas micro-grafts and indicating an outright graft incompatibility. Accumulation of phenol deposits at the union interfaces inhibited graft compatibility in many U. kirkiana combinations. Callus fusion technique can be used to identify partners with an outright graft incompatibility, especially for distant related plant species.
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Agufa CAC (2002) Genetic variation in Sclerocarya birrea and Uapaca kirkiana indigenous fruit trees of the miombo woodland. MSc thesis, Jomo Kenyatta University of Agriculture and Technology, 123 pp
Akinnifesi FK, Kwesiga FR, Mhango J, Mkonda A, Chilanga T, Swai R (2004) Domesticating priority for Miombo indigenous fruit trees as a promising livelihood option for small-holder farmers in Southern Africa. Acta Hortic 632:15–30
Akinnifesi FK, Kwesiga FR, Mhango J, Chilanga T, Mkonda A, Kadu CAC, Kadzere I, Mithöfer D, Saka JDK, Sileshi G, Ramadhani T, Dhliwayo P (2006) Towards the development of Miombo fruit trees as commercial tree crops in Southern Africa. For Trees Live 16:103–121
Akinnifesi FK, Mhango J, Sileshi G, Chilanga T (2007). Early growth and survival of three Miombo indigenous fruit tree species under fertilizer, manure and dry-season irrigation in southern Malawi. For Ecol Manage (DOI.10.1016/j.foreco.2007.09.025)
Considine JA (1983) Concepts and practice of use of plant growth regulating chemicals in viticulture. In: Nickell LG (ed) Plant growth regulating chemicals, vol 1. CRC Press Inc., Florida, pp 89–183
Errea P, Felipe A, Herrero M (1994) Graft establishment between compatible and incompatible Prunus spp. J Exp Bot 272:393–401
Errea P (1998) Implications of phenolic compounds in graft incompatibility in fruit tree species. Sci Hortic 74:195–205
Errea P, Garay L, Marin JA (2001) Early detection of graft incompatibility in apricot (Prunus armeniaca) using in vitro techniques. Physiol Plant 112:135–141
Ermel FF, Poessel JL, Faurobert M, Catesson AM (1997) Early scion/stock junction in compatible and incompatible pear/pear and pear/quince grafts: A histo-cytological study. Ann Bot 79:505–515
GenStat release 4.24DE (2005) GenStat for windows, discovery 2nd edn. Lawes Agricultural Trust, Rothamsted Experimental Station, UK
Ham C, Akinnifesi FK, Franzel F, Jordaan D du PS, Hansmann C, Ajayi OC, de Kock C (2007) Opportunities for Commercialization and Enterprise Development of Indigenous Fruits in southern Africa. In: Akinnifesi FK, Leakey RRB, Ajayi OC, Sileshi G, Tchoundjeu Z, Matakala P, Kwesiga FR (eds) Indigenous fruit trees in the Tropics: domestication, utilization and commercialization. World Agroforestry Centre: Nairobi. CAB International Publishing, Wallingford, UK, pp 254–272
Hamisy WC (2004) Promotion of effective conservation and sustainable utilization of Uapaca kirkiana Arg. Müel. Project Report, ICRAF Nairobi, Kenya
Jonard R, Lukman D, Schall F, Villemur P (1990) Early testing of graft incompatibilities in apricot and lemon trees using in vitro callus techniques. Sci Hortic 53:117–128
Kwesiga FR, Akinnifesi FK, Ramadhani T, Kadzere I, Saka JDK (2000) Domestication of indigenous fruit trees of the Miombo in southern Africa. In: Shumba EM, Lusepani E, Hangula R (eds) The domestication and commercialization of indigenous fruit trees in the SADC Region. SADC Tree Seed Centre Network, Harare, Zimbabwe, pp 8–24
Maghembe JA, Seyani JH (1991) Multipurpose trees used by smallholders in Malawi: results of ethnobotanical survey. AFRENA report No 42. ICRAF, Nairobi, Kenya, 30 pp
Maghembe JA, Simons AJ, Kwesiga F, Rarieya MM (1998) Selecting indigenous fruit trees for domestication in southern Africa. Nairobi, ICRAF, pp 1–39
Mithöfer D, Waibel H, Akinnifesi FK (2006) The role of food from natural resources in reducing vulnerability to poverty: a case study from Zimbabwe. Proceedings of the 26th conference on the International Association of Agricultural Economists (IAAE), August 12–26, Queensland, Australia
Moore R (1986) Graft incompatibility between pear and quince: the influence of metabolites of Pyrus communis. Am J Bot 73:1–4
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
Nito N, Han SH, Katayama Y (2005) Evaluation of graft compatibility for taxonomically relationships among species of the orange subfamily. Acta Hortic 692:85–89
Pina A, Errea P (2005) A review of new advances in mechanism of graft compatible–incompatible in Prunus spp. Sci Hortic 106:1–11
Ramadhani T (2002) Marketing of indigenous fruits in Zimbabwe. Socio-economic studies on rural development, vol. 129. Wissenchaftsverlag Vauk, Kiel, Germany
Sita GL, Raghava-Swamy BV (1993) Regeneration of plantlets from leaf disc cultures of rosewood: control of leaf abscission and shoot tip necrosis. Plant Sci 88:107–112
Financial support from the Federal Ministry of Economic Cooperation (BMZ/GTZ), Germany for through the World Agroforestry Centre Southern Africa Regional Programme is acknowledged.
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Mng’omba, S.A., du Toit, E.S. & Akinnifesi, F.K. Early recognition of graft compatibility in Uapaca kirkiana Müell Arg. clones, provenances and species. Agroforest Syst 74, 173–183 (2008). https://doi.org/10.1007/s10457-007-9100-7