Assessment of Ablative Therapies in Swine: Response of Respiratory Diaphragm to Varying Doses

  • Ashish Singal
  • Lars M. Mattison
  • Charles L. Soule
  • John R. Ballard
  • Eric N. Rudie
  • Erik N. K. Cressman
  • Paul A. Iaizzo
Article
  • 28 Downloads

Abstract

Ablation is a common procedure for treating patients with cancer, cardiac arrhythmia, and other conditions, yet it can cause collateral injury to the respiratory diaphragm. Collateral injury can alter the diaphragm’s properties and/or lead to respiratory dysfunction. Thus, it is important to understand the diaphragm’s physiologic and biomechanical properties in response to ablation therapies, in order to better understand ablative modalities, minimize complications, and maximize the safety and efficacy of ablative procedures. In this study, we analyzed physiologic and biomechanical properties of swine respiratory diaphragm muscle bundles when exposed to 5 ablative modalities. To assess physiologic properties, we performed in vitro tissue bath studies and measured changes in peak force and baseline force. To assess biomechanical properties, we performed uniaxial stress tests, measuring force–displacement responses, stress–strain characteristics, and avulsion forces. After treating the muscle bundles with all 5 ablative modalities, we observed dose-dependent sustained reductions in peak force and transient increases in baseline force—but no consistent dose-dependent biomechanical responses. These data provide novel insights into the effects of various ablative modalities on the respiratory diaphragm, insights that could enable improvements in ablative techniques and therapies.

Keywords

Radiofrequency ablation Cryoablation Microwave ablation High-intensity focused ultrasound ablation Chemical ablation 

Abbreviations

BF

Baseline force

CHA

Chemical ablation

CRA

Cryoablation

HCC

Hepatocellular carcinoma

HIFU

High-intensity focused ultrasound

MWA

Microwave ablation

PF

Peak force

RFA

Radiofrequency ablation

Notes

Acknowledgments

This study was supported by the Institute for Engineering in Medicine at the University of Minnesota, Medtronic, Minnesota Muscle Training Program Grant #2T32ST007612, and Minnesota Partnership for Biotechnology and Medical Genomics Grant #14.31. We gratefully acknowledge Mary Knatterud, Monica Mahre, and Dave Euler for reviewing the manuscript. Dr. Iaizzo has a research contract with Medtronic.

Supplementary material

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Copyright information

© Biomedical Engineering Society 2018

Authors and Affiliations

  1. 1.Departments of Surgery and Biomedical EngineeringUniversity of MinnesotaMinneapolisUSA
  2. 2.Institute for Engineering in MedicineUniversity of MinnesotaMinneapolisUSA

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