Annals of Biomedical Engineering

, Volume 46, Issue 5, pp 749–761 | Cite as

Investigating the Longitudinal Effect of Ovariectomy on Bone Properties Using a Novel Spatiotemporal Approach

Article
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Abstract

Osteoporosis is the most common bone disease. However, the mechanism of osteoporosis-induced alterations in bone is still unclear. The aim of this study was to investigate the effects of osteoporosis on the structural, densitometric and mechanical properties of the whole tibia using in vivo μCT imaging, spatiotemporal analysis and finite element modeling. Twelve C57Bl/6 female mice were adopted. At 14 weeks of age, half of the mice were ovariectomized (OVX), and the other half were SHAM-operated. The whole right tibia was scanned using an in vivo μCT imaging system at 14, 16, 17, 18, 19, 20, 21 and 22 weeks. The image datasets were registered in order to precisely quantify the bone properties. The results showed that OVX led to a significant increase in the endosteal area across the whole tibia 4 weeks after OVX intervention but did not have a significant influence on the periosteal area. Additionally, the bone volume and mineral content significantly decreased only in the proximal regions, but these decreases did not have a significant influence on the stiffness and failure load of the tibia. This study demonstrated the application of a novel spatiotemporal approach in the comprehensive analysis of bone adaptations in the spatiotemporal space.

Keywords

Osteoporosis Mouse tibia In vivo μCT Spatiotemporal analysis Finite element modeling 

Notes

Acknowledgments

This work was funded by the National Natural Science Foundation of China (11702057, 11772086), and the Open Fund from the State Key Laboratory of Structural Analysis for Industrial Equipment (GZ1611). The raw data analyzed and reported in this paper are obtained from the project funded by the UK National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs), grant number: NC/K000780/1. The raw data are available in Open Access under CC-BY-NC license and can be retrieved with the following  https://doi.org/10.15131/shef.data.3814701.

Conflict of interest

The authors declare that there are no financial or personal relationships with other persons or organizations that might inappropriately influence this work.

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Copyright information

© Biomedical Engineering Society 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Structural Analysis for Industrial EquipmentDalian University of TechnologyDalianChina
  2. 2.Department of Engineering MechanicsDalian University of TechnologyDalianChina
  3. 3.Department of Mechanical EngineeringNational University of SingaporeSingaporeSingapore
  4. 4.Department of Design Engineering and Mathematics, School of Science and TechnologyMiddlesex UniversityLondonUK

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