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Viscoelastic analysis of single-component and composite PEG and alginate hydrogels

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Abstract

Hydrogels, three-dimensional hydrophilic polymer networks, are appealing candidate materials for studying the cellular microenvironment as their substantial water content helps to better mimic soft tissue. However, hydrogels can lack mechanical stiffness, strength, and toughness. Composite hydrogel systems have been shown to improve upon mechanical properties compared to their singlecomponent counterparts. Poly (ethylene glycol) dimethacrylate (PEGDMA) and alginate are polymers that have been used to form hydrogels for biological applications. Singlecomponent and composite PEGDMA and alginate systems were fabricated with a range of total polymer concentrations. Bulk gels were mechanically characterized using spherical indentation testing and a viscoelastic analysis framework. An increase in shear modulus with increasing polymer concentration was demonstrated for all systems. Alginate hydrogels were shown to have a smaller viscoelastic ratio than the PEGDMA gels, indicating more extensive relaxation over time. Composite alginate and PEGDMA hydrogels exhibited a combination of the mechanical properties of the constituents, as well as a qualitative increase in toughness. Additionally, multiple hydrogel systems were produced that had similar shear moduli, but different viscoelastic behaviors. Accurate measurement of the mechanical properties of hydrogels is necessary in order to determine what parameters are key in modeling the cellular microenvironment.

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References

  1. Hoffman, A.S.: Hydrogels for biomedical applications. Annals of the New York Academy of Sciences 944, 62–73 (2001)

    Article  Google Scholar 

  2. Ratner, B.D., Hoffman, A.S.: Synthetic hydrogels for biomed ical applications. In Hydrogels for Medical and Related Applications, ACS Symposium Series 31, 1–36 (1976)

    Article  Google Scholar 

  3. Shapiro, J.M., Oyen, M.L.: Hydrogel composite materials for tissue engineering scaffolds. JOM 65, 1–12 (2013)

    Article  Google Scholar 

  4. Lutolf, M.P., Hubbell, J.A.: Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering. Nature Biotechnology 23, 47–55 (2005)

    Article  Google Scholar 

  5. Engler, A.J., Sen, S., Sweeney, H.L., et al.: Matrix elasticity directs stem cell lineage specification. Cell 126, 677–689 (2006)

    Article  Google Scholar 

  6. Trappmann, B., Gautrot, J.E., Connelly, J.T., et al.: Extracellular-matrix tethering regulates stem-cell fate. Nature Materials 11, 642–649 (2012)

    Article  Google Scholar 

  7. Oyen, M.L.: Mechanical characterisation of hydrogel materials. International Materials Reviews 59, 44–59 (2014)

    Article  Google Scholar 

  8. Baroli, B.: Hydrogels for tissue engineering and delivery of tissue-inducing substances. Journal of Pharmaceutical Sciences 96, 2197–2223 (2007)

    Article  Google Scholar 

  9. Rowley, J.A., Madlambayan, G., Mooney, D.J.: Alginate hydrogels as synthetic extracellular matrix materials. Biomaterials 20, 45–53 (1999)

    Article  Google Scholar 

  10. Burdick, J.A., Anseth, K.S.: Photoencapsulation of osteoblasts in injectable RGD-modified PEG hydrogels for bone tissue engineering. Biomaterials 23, 4315–4323 (2002)

    Article  Google Scholar 

  11. Zhu, J.: Bioactive modification of poly (ethylene glycol) hydrogels for tissue engineering. Biomaterials 31, 4639–4546 (2010)

    Article  Google Scholar 

  12. Galli, M., Comley, K.S.C., Shean, T.A.V., et al.: Viscoelastic and poroelastic mechanical characterization of hydrated gels. Journal of Materials Research 24, 973–979 (2009)

    Article  Google Scholar 

  13. Draget, K.I., Østgaard, K., Smidsrød, O.: Homogeneous alginate gels: A technical approach. Carbohydrate Polymers 14, 159–178 (1990)

    Article  Google Scholar 

  14. Johnson, K.L.: Contact Mechanics. Cambridge University Press, Cambridge (1985)

    Book  MATH  Google Scholar 

  15. Oyen, M.L.: Analytical techniques for indentation of viscoelastic materials. Philosophical Magazine 86, 5625–5641 (2006)

    Article  Google Scholar 

  16. Mattice, J.M., Lau, A.G., Oyen, M.L., et al.: Spherical indentation load-relaxation of soft biological tissues. Journal of Materials Research 21, 2003–2010 (2006)

    Article  Google Scholar 

  17. Lee, E.H., Radok, J.R.M.: The contact problem for viscoelastic bodies. Journal of Applied Mechanics 27, 438–444 (1960)

    Article  MATH  MathSciNet  Google Scholar 

  18. Discher, D.E., Janmey, P., Wang, Y.L.: Tissue cells feel and respond to the stiffness of their substrate. Science 310, 1139–1143 (2005)

    Article  Google Scholar 

  19. Discher, D.E., Mooney, D.J., Zandstra, P.W.: Growth factors, matrices, and forces combine and control stem cells. Science 324, 1673–1677 (2009)

    Article  Google Scholar 

  20. Lutolf, M.P., Raeber, G.P., Zisch, A.H., et al.: Cell-responsive synthetic hydrogels. Advanced Materials 15, 888–892 (2003)

    Article  Google Scholar 

  21. Oyen, M.L.: Poroelastic nanoindentation responses of hydrated bone. Journal of Materials Research 23, 1307–1314 (2008)

    Article  Google Scholar 

  22. Oyen, M.L., Shean, T.A.V., Strange, D.G.T., et al.: Size effects in indentation of hydrated biological tissues. Journal of Materials Research 27, 245–255 (2011)

    Article  Google Scholar 

  23. Hu, Y., Zhao, X., Vlassak, J.J., et al.: Using indentation to characterize the poroelasticity of gels. Applied Physics Letters 96, 121904 (2010)

    Article  Google Scholar 

  24. Cha, C., Kim, S.Y., Cao, L., et al.: Decoupled control of stiffness and permeability with a cell-encapsulating poly(ethylene glycol) dimethacrylate hydrogel. Biomaterials 31, 4864–4871 (2010)

    Article  Google Scholar 

  25. Yonet-Tanyeri, N., Rich, M.H., Lee, M., et al.: The spatiotemporal control of erosion and molecular release from micropatterned poly(ethylene glycol)-based hydrogel. Biomaterials 34, 8416–8423 (2013)

    Article  Google Scholar 

  26. Strange, D.G.T.: Mechanics of biomimetic materials for tissue engineering of the intervertebral disc, [PhD. Thesis]. University of Cambridge, Cambridge (2013)

    Google Scholar 

  27. LeRoux, M.A., Guilak, F., Setton, L.A.: Compressive and shear properties of alginate gel: Effects of sodium ions and alginate concentration. Journal of Biomedical Materials Research 47, 46–53 (1999)

    Article  Google Scholar 

  28. Strange, D.G.T., Oyen, M.L.: Composite hydrogels for nucleus pulposus tissue engineering. Journal of the Mechanical Behavior of Biomedical Materials 11, 16–26 (2012)

    Article  Google Scholar 

  29. Purslow, P.P. Measurement of the fracture toughness of extensible connective tissues. Journal of Materials Science 18, 3591–3598 (1983)

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Cambridge University Engineering Department, Cambridge, UK

    Jenna M. Shapiro

  2. Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA

    Jenna M. Shapiro

  3. Cambridge University Engineering Department, Cambridge, UK

    Michelle L. Oyen

Authors
  1. Jenna M. Shapiro
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  2. Michelle L. Oyen
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Correspondence to Michelle L. Oyen.

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Shapiro, J.M., Oyen, M.L. Viscoelastic analysis of single-component and composite PEG and alginate hydrogels. Acta Mech Sin 30, 7–14 (2014). https://doi.org/10.1007/s10409-014-0025-x

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  • DOI: https://doi.org/10.1007/s10409-014-0025-x

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