Abstract
The miniaturization of flow focuser is a challenge in developing microflow cytometers. Most previously reported microfluidic cell focusers require complex structures or external force fields to achieve the 3D cell focusing. Herein, we propose a novel circular-channel particle focuser utilizing viscoelastic focusing. The circular-channel particle focuser is fabricated using a simple and low-cost microwire molding technique. Whole PDMS channels with perfect circular cross-sections can be fabricated using this protocol. We then characterize the particle focusing performances in our circular-channel particle focuser and discuss the effects of particle size, operating flow rate, cross-sectional dimension and fluid rheological property on particle focusing. The experimental results show that a perfect single-line focusing can be achieved exactly at the channel centerline. Finally, our circular-channel particle focuser is employed for the focusing of blood cells. As it offers special advantages of simple structure, easy fabrication, and sheathless operation, our circular-channel particle focuser may serve as a potential flow focuser for microflow cytometers.
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References
Amini H, Lee W, Di Carlo D (2014) Inertial microfluidic physics. Lab Chip 14:2739–2761
Asghari M, Serhatlioglu M, Ortaç B, Solmaz ME, Elbuken C (2017) Sheathless microflow cytometry using viscoelastic fluids. Sci Rep 7:12342
Ateya DA, Erickson JS, Howell PB, Hilliard LR, Golden JP, Ligler FS (2008) The good, the bad, and the tiny: a review of microflow cytometry. Anal Bioanal Chem 391:1485–1498
Chabinyc ML, Chiu DT, McDonald JC, Stroock AD, Christian JF, Karger AM, Whitesides GM (2001) An integrated fluorescence detection system in poly (dimethylsiloxane) for microfluidic. Appl Anal Chem 73:4491–4498
Chang C-C, Huang Z-X, Yang R-J (2007) Three-dimensional hydrodynamic focusing in two-layer polydimethylsiloxane (PDMS) microchannels. J Micromech Microeng 17:1479
Chen Q et al (2018) Tunable, sheathless focusing of diamagnetic particles in ferrofluid microflows with a single set of overhead permanent magnets. Anal Chem 90:8600–8606
D’Avino G, Romeo G, Villone MM, Greco F, Netti PA, Maffettone PL (2012) Single line particle focusing induced by viscoelasticity of the suspending liquid: theory, experiments and simulations to design a micropipe flow-focuser. Lab Chip 12:1638–1645
D’Avino G, Greco F, Maffettone PL (2017) Particle migration due to viscoelasticity of the suspending liquid and its relevance in microfluidic devices. Annu Rev Fluid Mech 49:341–360
Del Giudice F, Greco F, Netti PA, Maffettone PL (2016) Is microrheometry affected by channel deformation? Biomicrofluidics 10:043501
Del Giudice F, Sathish S, D’Avino G, Shen AQ (2017) “From the edge to the center”: viscoelastic migration of particles and cells in a strongly shear-thinning liquid flowing in a microchannel. Anal Chem 89:13146–13159
Ding X et al (2013) Surface acoustic wave microfluidics. Lab Chip 13:3626–3649
Grosse A, Grewe M, Fouckhardt H (2001) Deep wet etching of fused silica glass for hollow capillary optical leaky waveguides in microfluidic devices. J Micromech Microeng 11:257
He F et al (2010) Fabrication of microfluidic channels with a circular cross section using spatiotemporally focused femtosecond laser pulses. Opt Lett 35:1106–1108
James DF (2009) Boger fluids. Ann Rev Fluid Mech 41:129–142
Jiang D, Tang W, Xiang N, Ni Z (2016) Numerical simulation of particle focusing in a symmetrical serpentine microchannel. RSC Adv 6:57647–57657
Kang K, Lee SS, Hyun K, Lee SJ, Kim JM (2013) DNA-based highly tunable particle focuser. Nat Commun 4:2567
Karimi A, Yazdi S, Ardekani AM (2013) Hydrodynamic mechanisms of cell particle trapping in microfluidics. Biomicrofluidics 7:021501
Karnis A, Mason SG (1966) Particle motions in sheared suspensions. XIX. Viscoelast Media Trans Soc Rheol 10:571–592
Kim JY, Ahn SW, Lee SS, Kim JM (2012) Lateral migration and focusing of colloidal particles and DNA molecules under viscoelastic flow. Lab Chip 12:2807–2814
Lee G-B, Chang C-C, Huang S-B, Yang R-J (2006) The hydrodynamic focusing effect inside rectangular microchannels. J Micromech Microeng 16:1024
Leshansky A, Bransky A, Korin N, Dinnar U (2007) Tunable nonlinear viscoelastic “focusing” in a microfluidic device. Phys Rev Lett 98:234501
Li D, Lu X, Xuan X (2016) Viscoelastic separation of particles by size in straight rectangular microchannels: a parametric study for a refined understanding. Anal Chem 88:12303–12309
Liu C, Xue C, Chen X, Shan L, Tian Y, Hu G (2015) Size-based separation of particles and cells utilizing viscoelastic effects in straight microchannels. Anal Chem 87:6041–6048
Liu C, Ding B, Xue C, Tian Y, Hu G, Sun J (2016) Sheathless focusing and separation of diverse nanoparticles in viscoelastic solutions with minimized shear. thinning. Anal Chem 88:12547–12553
Liu C et al (2017) Field-free isolation of exosomes from extracellular vesicles by microfluidic viscoelastic flows. ACS Nano 11:6968–6976
Lu X, Liu C, Hu G, Xuan X (2017) Particle manipulations in non-Newtonian microfluidics: a review. J Colloid Interface Sci 500:182–201
Muirhead KA, Horan PK, Poste G (1985) Flow cytometry: present and future. Bio/Technology 3:337
Nam J, Tan JKS, Khoo BL, Namgung B, Leo HL, Lim CT, Kim S (2015) Hybrid capillary-inserted microfluidic device for sheathless particle focusing and separation viscoelastic flow. Biomicrofluidics 9:064117
Otto O et al (2015) Real-time deformability cytometry: on-the-fly cell mechanical phenotyping. Nat Methods 12:199
Piyasena ME, Graves SW (2014) The intersection of flow cytometry with microfluidics and microfabrication. Lab Chip 14:1044–1059
Romeo G, D’Avino G, Greco F, Netti PA, Maffettone PL (2013) Viscoelastic flow-focusing in microchannels: scaling properties of the particle radial distributions. Lab Chip 13:2802–2807
Seo KW, Kang YJ, Lee SJ (2014) Lateral migration and focusing of microspheres in a microchannel flow of viscoelastic fluids. Phys Fluids 26:063301
Simonnet C, Groisman A (2005) Two-dimensional hydrodynamic focusing in a simple microfluidic device. Appl Phys Lett 87:114104
Sollier E, Murray C, Maoddi P, Di Carlo D (2011) Rapid prototyping polymers for microfluidic devices and high pressure injections. Lab Chip 11:3752–3765
Squires TM, Quake SR (2005) Microfluidics: fluid physics at the nanoliter scale. Rev Mod Phys 77:977
Steinkamp JA (1984) Flow cytometry. Rev Sci Instr 55:1375–1400
Sun T, Morgan H (2010) Single-cell microfluidic impedance cytometry: a review. Microfluid Nanofluid 8:423–443
Tian F, Cai L, Chang J, Li S, Liu C, Li T, Sun J (2018) Label-free isolation of rare tumor cells from untreated whole blood by interfacial viscoelastic microfluidics. Lab Chip 18:3436–3445
Wang G-J, Ho K-H, Hsu S-h, Wang K-P (2007) Microvessel scaffold with circular microchannels by photoresist melting. Biomed Microdev 9:657–663
Whitesides GM (2006) The origins and the future of microfluidics. Nature 442:368
Wilson ME, Kota N, Kim Y, Wang Y, Stolz DB, LeDuc PR, Ozdoganlar OB (2011) Fabrication of circular microfluidic channels by combining mechanical micromilling and soft lithography. Lab Chip 11:1550–1555
Wu W, DeConinck A, Lewis JA (2011) Omnidirectional printing of 3D microvascular networks. Adv Mater 23:H178-H183
Xia Y, Whitesides GM (1998) Soft lithography. Annu Rev Mater Sci 28:153–184
Xiang N, Shi Z, Tang W, Huang D, Zhang X, Ni Z (2015) Improved understanding of particle migration modes in spiral inertial microfluidic devices. RSC Adv 5:77264–77273
Xiang N, Dai Q, Ni Z (2016a) Multi-train elasto-inertial particle focusing in straight microfluidic channels. Appl Phys Lett 109:134101
Xiang N, Zhang X, Dai Q, Cheng J, Chen K, Ni Z (2016b) Fundamentals of elasto-inertial particle focusing in curved microfluidic channels. Lab Chip 16:2626–2635
Yan S et al (2018) Liquid metal-based amalgamation-assisted lithography for fabrication of complex channels with diverse structures and configurations. Lab Chip 18:785–792
Yang R, Feeback DL, Wang W (2005) Microfabrication and test of a three-dimensional polymer hydro-focusing unit for flow cytometry applications. Sens Actuators A Phys 118:259–267
Yang S, Kim JY, Lee SJ, Lee SS, Kim JM (2011) Sheathless elasto-inertial particle focusing and continuous separation in a straight rectangular microchannel. Lab Chip 11:266–273
Yang S et al (2012) Deformability-selective particle entrainment and separation in a rectangular microchannel using medium viscoelasticity. Soft Matter 8:5011–5019
Yuan D, Zhao Q, Yan S, Tang S-Y, Alici G, Zhang J, Li W (2018) Recent progress of particle migration in viscoelastic fluids. Lab Chip 18:551–567
Zhang J, Yan S, Sluyter R, Li W, Alici G, Nguyen N-T (2014) Inertial particle separation by differential equilibrium positions in a symmetrical serpentine micro-channel. Sci Rep 4:4527
Zhang J, Yan S, Yuan D, Alici G, Nguyen N-T, Warkiani ME, Li W (2016) Fundamentals and applications of inertial microfluidics: a review. Lab Chip 16:10–34
Zhao Y, Fujimoto BS, Jeffries GDM, Schiro PG, Chiu DT (2007) Optical gradient flow focusing. Opt Express 15:6167–6176
Zhu J, Tzeng T-RJ, Hu G, Xuan X (2009) DC dielectrophoretic focusing of particles in a serpentine microchannel. Microfluid Nanofluid 7:751
Acknowledgements
This research work is supported by the National Natural Science Foundation of China (81727801, 51875103, 51505082, and 51775111), the Natural Science Foundation of Jiangsu Province (BK20150606), the Fundamental Research Funds for the Central Universities (2242017K41031), the Six Talent Peaks Project of Jiangsu Province (SWYY-005) and the ZhiShan Young Scholar Fellowship.
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This article is part of the topical collection “Particle motion in non-Newtonian microfluidics” guest edited by Xiangchun Xuan and Gaetano D’Avino.
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Xiang, N., Dai, Q., Han, Y. et al. Circular-channel particle focuser utilizing viscoelastic focusing. Microfluid Nanofluid 23, 16 (2019). https://doi.org/10.1007/s10404-018-2184-8
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DOI: https://doi.org/10.1007/s10404-018-2184-8