Immunochromatographic thread-based test platform for diagnosis of infectious diseases
- 228 Downloads
Patterning is an important step in fabrication of multiplexed microfluidic devices. Various approaches including cutting, photolithography, wax-printing, plotting and etching have been developed and tested. Recently, using threads has emerged as a convenient and low-cost approach for fabrication of microfluidic devices. We explored the application of threads in combination with nitrocellulose membrane to fabricate multi-channel immunochromatographic diagnostic devices. Microfluidic channels were made using hydrophilic threads and nitrocellulose membrane strips. Household sewing needle was used to weave hydrophilic thread into desired patterns through a double-sided mounting tape. Glass fibre discs were used as conjugate pads while nitrocellulose membrane was used for immobilisation of capture antibodies. Patterned threads were linked to nitrocellulose membrane strips by overlapping so that reagents flowing through threads were eventually transferred to the membrane. The design was tested using IgG, H. pylori and Hepatitis B surface antigen. Continuous flow was observed from hydrophilic threads to the nitrocellulose membrane, and a positive signal was visualised on the membrane within 5 min of sample application. The observed limit of detection ranged between 30 and 300 ng/ml for H. pylori and Hepatitis B, respectively. Using thread and tape offers a promising alternative for patterning of simple, low-cost multiplexed microfluidic diagnostic devices with potential point-of-care applications in resource-limited settings.
KeywordsImmunochromatographic Thread-based diagnostics Infectious diseases Multiplex microfluidic devices Point-of-care Low-cost diagnostics
We thank the technical staff at NM-AIST for their cooperation and support during execution of this work. This work was supported by the Tanzania Commission for Science and Technology (COSTECH) through the Nelson Mandela African Institution of Sciences and Technology (NM-AIST) Graduate Scholarship.
MS and JB developed the research plan. MS and DM carried out research work with the help of JB. MS interpreted the data and prepared the manuscript. DM and JB edited and reviewed the manuscript. All authors read and approved the final version of the manuscript.
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflict of interest.
- Law JWF, Ab Mutalib NS, Chan KG, Lee LH (2014) Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations. Front Microbiol 5:770Google Scholar
- Sicard C, Gien C, Aubie B, Wallace D, Jahanshahi-Anbuhi S, Pennings K, Daigger GT, Pelton R, Brennan JD, Fillipe CD (2015) Tools for water quality monitoring and mapping using paper-based sensors and cell phones. Water Res 2015(70):360–369. https://doi.org/10.1016/j.watres.2014.12.005 CrossRefGoogle Scholar