Inertial particle focusing and spacing control in microfluidic devices

  • Chao Wang
  • Sifan Sun
  • Ying Chen
  • Zhengdong Cheng
  • Yuxiu Li
  • Lisi Jia
  • Pengcheng Lin
  • Zhi Yang
  • Riyang Shu
Research Paper
  • 202 Downloads

Abstract

Focusing particles into a tight stream is usually a necessary step prior to counting, detecting and sorting them. Meanwhile, particle spacing control in microfluidic devices could also be applied in the field of accurate cell detection, material synthesis and chemical reaction. To achieve simultaneous particle focusing and spacing control, a novel microchannel composed by Dean and sheath flow section was proposed and fabricated according to the elaborated design principle with its manipulating performance in situ visualized. Using microspheres with a few microns as a template, the trajectory of the particles was discovered to follow lateral migration and reach certain equilibrium positions at the end of the designed Dean section. After being focused, the streamline was further concentrated and centralized with a controllable interparticle distance in sheath flow section. For sheath flow section, the angle between symmetrical tributaries and the mainstream channel and abrupt constriction/expansion structure of mainstream channel as important channel geometric features were investigated to minimize the focusing streamline width and optimize spacing control. An modified analytical model for sheath flow with different tributary angles was derived and proved to well describe the microsphere spacing control process.

Keywords

Microfluidics Sheath flow Dean flow Enhancing inertial focusing Spacing control 

Notes

Acknowledgements

This work was supported by Natural Science Foundation of China (No. 51606046), Research and Development Project for Application supported by Guangdong (2016B020243010) and International Scientific and Technological Cooperation Project by Guangzhou city (2016201604030063).

Compliance with ethical standards

Conflict of interest

The authors declared that they have no conflict of interest.

Supplementary material

10404_2018_2035_MOESM1_ESM.docx (19 kb)
Supplementary material 1 (DOCX 19 kb)

References

  1. Asmolov ES (1999) The inertial lift on a spherical particle in a plane Poiseuille flow at large channel Reynolds number. J Fluid Mech 381:63–87CrossRefMATHGoogle Scholar
  2. Berger SA, Talbot L, Yao LS (2003) Flow in curved pipes. Annu Rev Fluid Mech 15:461–512CrossRefMATHGoogle Scholar
  3. Bhagat AAS, Kuntaegowdanahalli SS, Papautsky I (2008a) Continuous particle separation in spiral microchannels using Dean flows and differential migration. Lab Chip 8:1906–1914CrossRefGoogle Scholar
  4. Bhagat AAS, Kuntaegowdanahalli SS, Papautsky I (2008b) Enhanced particle filtration in straight microchannels using shear-modulated inertial migration. Phys Fluids 20:101702CrossRefMATHGoogle Scholar
  5. Bhagat AAS, Kuntaegowdanahalli SS, Papautsky I (2009) Inertial microfluidics for continuous particle filtration and extraction. Microfluid Nanofluid 7:217–226CrossRefGoogle Scholar
  6. Cartas-Ayala MA, Raafat M, Karnik R (2013) Microfluidic circuits: self-sorting of deformable particles in an asynchronous logic microfluidic circuit (Small 3/2013). Small 9:333CrossRefGoogle Scholar
  7. Dean WR (1928) Fluid motion in a curved channel. Proc R Soc Lond Ser A 121:402–420CrossRefMATHGoogle Scholar
  8. Di Carlo D (2009) Inertial microfluidics. Lab Chip 9:3038–3046CrossRefGoogle Scholar
  9. Di Carlo D, Irimia D, Tompkins RG, Toner M (2007) Continuous inertial focusing, ordering, and separation of particles in microchannels. Proc Natl Acad Sci 104:18892–18897CrossRefGoogle Scholar
  10. Di Carlo D, Edd JF, Irimia D, Tompkins RG, Toner M (2008) Equilibrium separation and filtration of particles using differential inertial focusing. Anal Chem 80:2204–2211CrossRefGoogle Scholar
  11. Eichhorn R, Small S (1964) Experiments on the lift and drag of spheres suspended in a Poiseuille flow. J Fluid Mech 20:513–527CrossRefMATHGoogle Scholar
  12. Faivre M, Abkarian M, Bickraj K, Stone HA (2006) Geometrical focusing of cells in a microfluidic device: an approach to separate blood plasma. Biorheology 43:147–159Google Scholar
  13. Ho BP, Leal LG (1974) Inertial migration of rigid spheres in two-dimensional unidirectional flows. J Fluid Mech 65:365–400CrossRefMATHGoogle Scholar
  14. Jiang H, Weng X, Li D (2014) A novel microfluidic flow focusing method. Biomicrofluidics 8:054120CrossRefGoogle Scholar
  15. Jones SW, Thomas OM, Aref H (1989) Chaotic advection by laminar flow in a twisted pipe. J Fluid Mech 209:335–357MathSciNetCrossRefGoogle Scholar
  16. Kemna EWM, Schoeman RM, Wolbers F, Vermes I, Weitz DA, Van Den Berg A (2012) High-yield cell ordering and deterministic cell-in-droplet encapsulation using Dean flow in a curved microchannel. Lab Chip 12:2881–2887CrossRefGoogle Scholar
  17. Knight JB, Vishwanath A (1998) Hydrodynamic focusing on a silicon chip: mixing nanoliters in microseconds. Phys Rev Lett 80:3863–3866CrossRefGoogle Scholar
  18. Kobayashi J, Mori Y, Okamoto K, Akiyama R, Ueno M, Kitamori T, Kobayashi S (2004) A microfluidic device for conducting gas–liquid–solid hydrogenation reactions. Science 304:1305–1308CrossRefGoogle Scholar
  19. Kummrow A, Theisen J, Frankowski M, Tuchscheerer A, Yildirim H, Brattke K, Schmidt M, Neukammer J (2009) Microfluidic structures for flow cytometric analysis of hydrodynamically focussed blood cells fabricated by ultraprecision micromachining. Lab Chip 9:972–981CrossRefGoogle Scholar
  20. Kuntaegowdanahalli SS, Bhagat AAS, Kumar G, Papautsky I (2009) Inertial microfluidics for continuous particle separation in spiral microchannels. Lab Chip 9:2973–2980CrossRefGoogle Scholar
  21. Leal LG (1980) Particle motions in a viscous fluid. Annu Rev Fluid Mech 12:435–476MathSciNetCrossRefMATHGoogle Scholar
  22. Lee MG, Choi S, Park JK (2009) Three-dimensional hydrodynamic focusing with a single sheath flow in a single-layer microfluidic device. Lab Chip 9:3155–3160CrossRefGoogle Scholar
  23. Lee W, Amini H, HA Stone, Di Carlo D (2010) Dynamic self-assembly and control of microfluidic particle crystals. Proc Natl Acad Sci 107:22413–22418CrossRefGoogle Scholar
  24. MacDonald MP, Spalding GC, Dholakia K (2003) Microfluidic sorting in an optical lattice. Nature 426:421–424CrossRefGoogle Scholar
  25. Mach AJ, Di Carlo D (2010) Continuous scalable blood filtration device using inertial microfluidics. Biotechnol Bioeng 107:302–311CrossRefGoogle Scholar
  26. Matas JP, Morris JF, Guazzelli É (2004a) Inertial migration of rigid spherical particles in Poiseuille flow. J Fluid Mech 515:171–195CrossRefMATHGoogle Scholar
  27. Matas JP, Glezer V, Guazzelli É, Morris JF (2004b) Trains of particles in finite-Reynolds-number pipe flow. Phys Fluids 16:4192–4195CrossRefMATHGoogle Scholar
  28. Nasir M, Ateya DA, Burk D, Golden JP, Ligler FS (2010) Hydrodynamic focusing of conducting fluids for conductivity-based biosensors. Biosens Bioelectron 25:1363–1369CrossRefGoogle Scholar
  29. Ookawara S, Street D, Ogawa K (2006) Numerical study on development of particle concentration profiles in a curved microchannel. Chem Eng Sci 61:3714–3724CrossRefGoogle Scholar
  30. Park JS, Jung HI (2009) Multiorifice flow fractionation: continuous size-based separation of microspheres using a series of contraction/expansion microchannels. Anal Chem 81:8280–8288CrossRefGoogle Scholar
  31. Park JS, Song SH, Jung HI (2008) Continuous focusing of microparticles using inertial lift force and vorticity via multi-orifice microfluidic channels. Lab Chip 9:939–948CrossRefGoogle Scholar
  32. Sajeesh P, Manasi S, Doble M, Sen AK (2015) A microfluidic device with focusing and spacing control for resistance-based sorting of droplets and cells. Lab Chip 15:3738–3748CrossRefGoogle Scholar
  33. Sudarsan AP, Ugaz VM (2005) Fluid mixing in planar spiral microchannels. Lab Chip 6:74–82CrossRefGoogle Scholar
  34. Sudarsan AP, Ugaz VM (2006) Multivortex micromixing. Proc Natl Acad Sci 103:7228–7233CrossRefGoogle Scholar
  35. Toner M, Di Carlo D, Edd JF, Irimia D (2014) Systems and methods for particle focusing in microchannels. US Patent 8,784,012Google Scholar
  36. Tripathi S, Kumar A, Kumar YVBV, Agrawal A (2016) Three-dimensional hydrodynamic flow focusing of dye, particles and cells in a microfluidic device by employing two bends of opposite curvature. Microfluid Nanofluid 20:34CrossRefGoogle Scholar
  37. Wang H, Sobahi N, Han A (2017) Impedance spectroscopy-based cell/particle position detection in microfluidic systems. Lab Chip 17:1264–1269CrossRefGoogle Scholar
  38. Whitesides GM (2006) The origins and the future of microfluidics. Nature 442:368–373CrossRefGoogle Scholar
  39. Xuan X, Zhu J, Church C (2010) Particle focusing in microfluidic devices. Microfluid Nanofluid 9:1–16CrossRefGoogle Scholar
  40. Zhou J, Papautsky I (2013) Fundamentals of inertial focusing in microchannels. Lab Chip 13:1121–1132CrossRefGoogle Scholar
  41. W. Lee, H. Amini, H. A. Stone, D. Di Carlo, (2010) Dynamic self-assembly and control of microfluidic particle crystals. Proceedings of the National Academy of Sciences 107 (52):22413-22418CrossRefGoogle Scholar
  42. Stillwell MT, Holdich RG, Kosvintsev SR, Gasparini G, Cumming IW (2007) Stirred cell membrane emulsification and factors influencing dispersion drop size and uniformity. Ind Eng Chem Res 46:965–972CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Chao Wang
    • 1
  • Sifan Sun
    • 1
  • Ying Chen
    • 1
  • Zhengdong Cheng
    • 2
  • Yuxiu Li
    • 1
  • Lisi Jia
    • 1
  • Pengcheng Lin
    • 1
  • Zhi Yang
    • 1
  • Riyang Shu
    • 1
  1. 1.Guangdong Provincial Key Laboratory on Functional Soft Condensed Matter, School of Materials and EnergyGuangdong University of TechnologyGuangzhouChina
  2. 2.Artie McFerrin Department of Chemical EngineeringTexas A&M UniversityCollege StationUSA

Personalised recommendations