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The flow resistance of single DNA-grafted colloids as measured by optical tweezers

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Abstract

In rheological experiments, the flow resistance of single blank or DNA-grafted colloids is determined and compared. The length of the double-stranded-DNA varies amongst 1,000, 4,000 and 6,000 base pairs (bp) corresponding to contour lengths varying between 340 and 2,040 nm at a grafting density of 0.03±0.01 μm2/chain. The degree of swelling of the grafted DNA is adjusted by exchanging the ion concentration of the surrounding medium. For all examined flow velocities ranging between 100 and 1,200 μm/s, one observes an interesting deviation from Stokes law which can be traced back to a shear-dependent conformational change of the brush layer. The ratio of the effective hydrodynamic radii of DNA-grafted and blank colloids shows a pronounced dependence on the flow velocity, but as well on the length of the grafted DNA and the ionic strength of the solvent. The experimental findings are in qualitative agreement with hydrodynamic simulations based on an elastically jointed chain model.

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References

  • Ashkin A (1997) Optical trapping and manipulation of neutral particles using Lasers. Proc Natl Acad Sci USA 94:4853–4860

    Article  PubMed  Google Scholar 

  • Bishop AI, Nieminen TT, Heckenberg NR, Rubensztein-Dunlop H (2004) Optical microrheology using rotating laser trapped particles.” Phys Rev Lett 92(19):198104

    Article  PubMed  Google Scholar 

  • Brochard-Wyart F (1993) Deformations of one tetheres chain in stranog flows. Europhys Lett 23:105–111

    Article  Google Scholar 

  • Brochard-Wyart F, Herver H, Pincus P (1994) Unwinding of polymer chains under forces or flows. Europhys Lett 26:511–516

    Article  Google Scholar 

  • Debye P (1908) Der Lichtdruck auf Kugeln von beliebigem Materia. Dissertation

  • Hapel J, Brenner H (1973) Low Reynolds number hydrodynamics: with special applications to particulate media. Noordhoff International Publishing, Leyden, pp 119–23

    Google Scholar 

  • Harden JL, Cates ME (1996) Phys Rev E f 53:3782

    Google Scholar 

  • Kim YW, Netz RR (2005) Europhys Lett 72:837

  • Kreer T, Binder K, Müser M (2003) Langmuir 19:7551

    Article  Google Scholar 

  • Langowski J (1987) Salt effects on internal motions of superhelical and linear pUC8 DNA Dynamic light scattering studies. Biophys Chem 27:263–271

    Article  PubMed  Google Scholar 

  • Ovryn B (2000) Three-dimensional forward scattering particle image velocimetry applied to a microscopic field-of-view. Exp Fluids 29:S175–S184

    Article  Google Scholar 

  • Ovryn B, Izen S (2000) Imaging of transparent spheres through a planar interface using a high-numerical-aperture optical microscope. J Opt Soc Am A 17:1202–1213

    Article  Google Scholar 

  • Schlagberger X, Netz RR (2005) Europhys Lett 70:129, 563

    Google Scholar 

  • van de Ven TGM (1989) Colloidal hydrodynamics. In: Colloid Science: a series of monographs. Academic, New York

Download references

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Authors and Affiliations

  1. Institut für Experimentelle Physik I der Universität Leipzig, Leipzig, Germany

    C. Gutsche & F. Kremer

  2. Institut für Biochemie der Universität Leipzig, Leipzig, Germany

    M. Salomo

  3. Physik Department, TU München, 85748, Garching, Germany

    Y. W. Kim & R. R. Netz

  4. Materials Research Laboratory, University of California at Santa Barbara, Santa Barbara, CA, 93106, USA

    Y. W. Kim

Authors
  1. C. Gutsche
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  2. M. Salomo
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  3. Y. W. Kim
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  4. R. R. Netz
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  5. F. Kremer
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Corresponding author

Correspondence to F. Kremer.

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Gutsche, C., Salomo, M., Kim, Y.W. et al. The flow resistance of single DNA-grafted colloids as measured by optical tweezers. Microfluid Nanofluid 2, 381–386 (2006). https://doi.org/10.1007/s10404-006-0080-0

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  • DOI: https://doi.org/10.1007/s10404-006-0080-0

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