Japanese Journal of Ophthalmology

, Volume 62, Issue 4, pp 473–480 | Cite as

Reliability of a commercially available heterochromatic flicker photometer, the MPS2, for measuring the macular pigment optical density of a Japanese population

  • Akira Obana
  • Yuko Gohto
  • Takatoshi Moriyama
  • Takahiko Seto
  • Hiroyuki Sasano
  • Shigetoshi Okazaki
Clinical Investigation



The macular pigment optical density (MPOD) of a Japanese population was measured using a commercially based heterochromatic flicker photometer, the Macular Pigment Screener (MPS2). The objective of the study was to evaluate the accuracy and test-retest reliability of the MPS2 in Asian pigmented eyes.

Study design

Experimental study to validate the medical instrument in humans.


Twenty-four healthy Japanese participants with no systemic or eye diseases (men: 13, women: 11; mean [SD] age 38.6 [10.9 years]) were included. The concordance of the MPOD, obtained using the MPS2 and Macular Metrics II (MM2), and the test-retest reliability were examined.


Determination of the MPOD was unsuccessful in 1 participant; thus, the MPOD of 23 participants was analyzed. The mean (SD) MPOD measured with the detail-mode of the MPS2 was 0.63 (0.18) and with that of the MM2, it was 0.72 (0.23). The former was significantly lower than the latter (P = .003, paired t test). The MPOD measured with the MPS2 and the MM2 showed good concordance (r = 0.79, P < .001, Pearson product moment correlation). Bland-Altman analyses showed no systematic errors between the MPS2 and the MM2. The intraclass correlation coefficient over 5 measurement times with the detail-mode of the MPS2 was 0.80, and the mean coefficient of variation was 9.4%.


The high concordance with the MM2 and good test-retest reliability found by this study suggest that the MPS2 is acceptable for use in a Japanese population. However, the mean MPOD yielded by the MPS2 was significantly lower than that yielded by the MM2. Therefore, the MPS2 and MM2 are not interchangeable in a single study.


Asian pigmented eyes Heterochromatic flicker photometry Macular pigment optical density MPS2 validation 



The authors would like to thank Takayuki Hosokawa (Department of Information Systems, Seirei Hamamatsu General Hospital) for his statistical support. No financial disclosures.


No funding support received.

Conflicts of interest

A. Obana, Equipment (ME Technica); Y. Gohto, Equipment (ME Technica); T. Moriyama, Equipment (ME Technica); T. Seto, Equipment (ME Technica); H. Sasano, Equipment (ME Technica); S. Okazaki, None.

Proprietary interest

Akira Obana, None; Yuko Gohto, None; Takahiko Seto, None; Hiroyuki Sasano, None; Takatoshi Moriyama, None; Shigetoshi Okazaki, None


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Copyright information

© Japanese Ophthalmological Society 2018

Authors and Affiliations

  • Akira Obana
    • 1
    • 2
  • Yuko Gohto
    • 1
  • Takatoshi Moriyama
    • 1
  • Takahiko Seto
    • 1
  • Hiroyuki Sasano
    • 1
  • Shigetoshi Okazaki
    • 2
  1. 1.Department of OphthalmologySeirei Hamamatsu General HospitalHamamatsu CityJapan
  2. 2.Department of Medical Spectroscopy, Institute for Medical Photonics Research, Preeminent Medical Photonics Education and Research CenterHamamatsu University School of MedicineHamamatsuJapan

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