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Exogenous Silicon Protects Brassica napus Plants from Salinity-Induced Oxidative Stress Through the Modulation of AsA-GSH Pathway, Thiol-Dependent Antioxidant Enzymes and Glyoxalase Systems

Exogenes Silizium schützt Brassica-napus-Pflanzen vor salzinduziertem oxidativen Stress durch die Modulation des AsA-GSH-Zyklus, thiolabhängiger antioxidativer Enzyme und der Glyoxalase-Systeme

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Abstract

Although silicon (Si) has showed its potential role in mitigating abiotic stress-induced damages in many plant species its role in coordinated induction of antioxidant defense is yet to be elucidated. Therefore, we studied rapeseed (Brassica napus) seedlings applied with exogenous Si for changes occurring in antioxidant defense and glyoxalase systems. Seedlings (12-day-old) grown semi-hydroponically were exposed to Si (silicon dioxide, SiO2; 1 mM) solely and in combination with NaCl (100 and 200 mM) for 48 h. Salinity created oxidative damage by increasing H2O2 and malondialdehyde (MDA) contents resulting in disruption of antioxidant defense system and in arousing methylglyoxal (MG) toxicity by the down-regulation of glyoxalase enzyme activities. Exogenous Si treatment showed reduction of both H2O2 and MDA contents and up-regulation of antioxidant components including the activities of related enzymes (APX, MDHAR, DHAR, GR, GST, GPX and CAT) and the contents of AsA and GSH. Enhanced activities of glyoxalase I (Gly I) and glyoxalase II (Gly II) detoxified the toxic MG. Thus, this study clearly indicates that Si improved plant tolerance to salinity stress through enhancement of both antioxidant defense and glyoxalase systems that led to reduced oxidative damage and MG toxicity.

Zusammenfassung

Obwohl Silizium (Si) seine potenzielle Rolle bei der Abschwächung abiotischer stressinduzierter Schäden bei vielen Pflanzenarten gezeigt hat, ist seine Rolle bei der koordinierten Induktion der antioxidativen Abwehr noch zu klären. Daher untersuchten wir Rapskeimlinge (Brassica napus), die mit exogenem Si behandelt wurden, auf Veränderungen in der antioxidativen Abwehr und der Glyoxalase-Systeme. Keimlinge (12 Tage alt), die semi-hydroponisch gezüchtet wurden, wurden 48 h Si (Siliciumdioxid, SiO2; 1 mM) ausgesetzt – allein und in Kombination mit NaCl (100 und 200 mM). Die Salinität verursachte oxidative Schäden durch die Erhöhung des H2O2- und Malondialdehyd (MDA)-Gehalts, was zu einer Störung des antioxidativen Abwehrsystems und zur Stimulation der Methylglyoxal (MG)-Toxizität durch die Herunterregulierung der Glyoxalase-Enzymaktivitäten führte. Die exogene Si-Behandlung zeigte eine Reduktion der H2O2- und der MDA-Gehalte sowie eine Hochregulierung der antioxidativen Komponenten einschließlich der Aktivitäten verwandter Enzyme (APX, MDHAR, DHAR, GR, GST, GPX und CAT) und der AsA- und GSH-Gehalte. Verstärkte Aktivitäten von Glyoxalase I (Gly I) und Glyoxalase II (Gly II) entgifteten das toxische MG. Somit zeigt diese Studie deutlich, dass Si die Toleranz von Pflanzen gegenüber Salzstress durch die Steigerung der antioxidativen Abwehr und der Aktivität der Glyoxalase-Systeme verbesserte, was zu einer verringerten oxidativen Schädigung und MG-Toxizität führte.

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Acknowledgements

We acknowledge Ms. Khursheda Parvin, Assistant Professor, Department of Horticulture, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh for the critical reading and editing of the manuscript.

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Authors and Affiliations

  1. Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, 1207, Dhaka, Bangladesh

    Mirza Hasanuzzaman & T. I. Anee

  2. Department of Agricultural Botany, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, 1207, Dhaka, Bangladesh

    K. Nahar

  3. Molecular Breeding Laboratory, Plant Breeding Division, Bangladesh Agricultural Research Institute, 1701, Gazipur, Bangladesh

    M. M. Rohman

  4. College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China

    Y. Huang

  5. Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, 761-0795, Kagawa, Japan

    M. Fujita

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  1. Mirza Hasanuzzaman
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Correspondence to Mirza Hasanuzzaman.

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M. Hasanuzzaman, K. Nahar, M.M. Rohman, T.I. Anee, Y. Huang and M. Fujita declare that they have no competing interests.

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Hasanuzzaman, M., Nahar, K., Rohman, M.M. et al. Exogenous Silicon Protects Brassica napus Plants from Salinity-Induced Oxidative Stress Through the Modulation of AsA-GSH Pathway, Thiol-Dependent Antioxidant Enzymes and Glyoxalase Systems. Gesunde Pflanzen 70, 185–194 (2018). https://doi.org/10.1007/s10343-018-0430-3

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