Influence of Bagging on Color, Anthocyanin and Anthocyanin Biosynthetic Genes in Peel of Red Colored Mango Cv. ‘Lily’

  • G. R. Karanjalker
  • K. V. Ravishankar
  • K. S. Shivashankara
  • M. R. Dinesh
Original Article
  • 47 Downloads

Abstract

Sunlight influences many morphological, biochemical and molecular changes in fruits and the most important is color change associated with the development of pigments. The exposure to sunlight influences the anthocyanin content of the fruit peel. With the aim to understand the influence of bagging of fruits on the mango peel color, anthocyanin and expression of anthocyanin biosynthetic genes, an experiment was conducted by covering fruits of red colored cv. ‘Lily’ with muslin cloth. The observations were recorded for fruits of control (non-bagged) and fruits developing in shade. In bagged fruits, the color of peel changed towards lightness and yellowness. The reduction in anthocyanin was observed up to 17 times in bagged and shaded fruits. We also observed the down-regulation of all anthocyanin biosynthetic genes in fruits under bagged and shaded conditions. This study reports the influence of bagging and shade on anthocyanin biosynthetic genes and subsequent change in the color of fruit peel.

Keywords

Mangifera indica L. Peel Bagging Color Total anthocyanin Gene expression 

Abbreviations

CHS

Chalcone synthase

CHI

Chalcone isomerase

F3H

Flavanone 3‑hydroxylase

F3,5H

Flavonoid 3,5-hydroxylase

ANS

Anthocyanin synthase

DFR

Dihydroflavonol-4-reductase

UFGT

UDP-glucose: flavonoid-O-glycosyl-transferase

BHLHX

Basic helix loop helix, MYB Transcription factor and WD-40R

Einfluss der Beschattung auf Farbe, Anthocyan und Anthocyan-Biosynthese-Gene der Fruchthaut bei rotschaligen Mangos der Sorte ‘Lily’

Schlüsselwörter

Mangifera indica L. Beschattung Farbe Gesamt-Anthocyan Genexpression Mango 

Notes

Acknowledgements

GRK thank University Grant Commission, New Delhi for providing Rajiv Gandhi National Fellowship (RGNF-ST-GOA-7140). Authors acknowledge the financial assistance from ICAR Network Project on Transgenics in Crops (NPTC): Functional genomics of plant type, maturity and fruit quality traits in Mango. Also, Dr. D. V. Sudhakar Rao (Principal Scientist, ICAR-IIHR, Bengaluru) is acknowledged for providing color reader.

Conflict of interest

G.R. Karanjalker, K.V. Ravishankar, K.S. Shivashankara and M.R. Dinesh declare that they have no competing interests.

References

  1. Bible BB, Singha S (1993) Canopy position influences CIELAB coordinates of peach color. HortScience 28:992–993Google Scholar
  2. Borsani O, Gonzalez-Neves G, Ferrer M, Monza J (2010) Anthocyanins accumulation and genes-related expression in berries of cv. tannat (vitis vinifera L.). J Appl Hortic 12(1):3–9Google Scholar
  3. Chen CS, Di Z, Ya-Qiang W, Peng-Min L, And Feng-Wang M (2012) Effects of fruit bagging on the contents of phenolic compounds in the peel and flesh of ‘Golden Delicious’, ‘Red Delicious’, and ‘Royal Gala’ apples. Sci Hortic 14:68–73CrossRefGoogle Scholar
  4. Genard M, Bruchou C (1992) Multivariate analysis of within-tree factors accounting for the variation of peach fruit quality. Sci Hortic 52(1):37–51CrossRefGoogle Scholar
  5. Hofman PJ, Smith LG, Joyce DC, Johnson GI, Meburg GF (1997) Bagging of mango (Mangifera indica cv. ‘Keitt’) fruit influences fruit quality and mineral composition. Postharvest Biol Technol 12:83–91CrossRefGoogle Scholar
  6. Huang ZL, Wang BW, Williams P, Pace RD (2009) Identification of anthocyanins in muscadine grapes with HPLC-ESI-MS. LWT Food Sci Technol 42:819–824CrossRefGoogle Scholar
  7. Hwang AS, Huang KL, Hsu SH (2004) Effect of bagging with black paper on coloration and fruit quality of ‘Ruby’ grapefruit. J Agric Res China 53:229–238Google Scholar
  8. Jaakola L (2013) New insights into the regulation of anthocyanin biosynthesis in fruits. Trends Plant Sci 18(9):477–483.  https://doi.org/10.1016/j.tplants.2013.06.003 CrossRefPubMedGoogle Scholar
  9. Jain M, Aashima N, Akhilesh KT, Jitendra PK (2006) Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR. Biochem Biophys Res Commun 345:646–651CrossRefPubMedGoogle Scholar
  10. Jia H, Araki A, Okamoto G (2005) Influence of fruit bagging on aroma volatiles and skin coloration of ‘Hakuho’ peach (Prunus persica Batsch). Postharvest Biol Technol 35:61–68CrossRefGoogle Scholar
  11. Jing C, Ma C, Zhang J, Jing S, Jiang X, Yang Y, Zao Z (2016) Effect of debagging time on delicious’ and ‘qinguan’ apple fruit at mid and late stages of development. PLoS ONE 11(10):e165050.  https://doi.org/10.1371/journal.pone.0165050 CrossRefPubMedPubMedCentralGoogle Scholar
  12. John AT, Hofman PJ, Underhill SJ, Bell KL (1998) Fruit canopy position and panicle bagging affects yield and quality of ‘tai so’ lychee. Sci Hortic 72:203–213CrossRefGoogle Scholar
  13. Ju ZG (1998) Fruit bagging, a useful method for studying anthocyanin synthesis and gene expression in apple. Sci Hortic 77:155–164CrossRefGoogle Scholar
  14. Kader AA (1999) Fruit maturity, ripening, and quality relationships. Acta Hortic 485:203–208CrossRefGoogle Scholar
  15. Kawphaitoon S, Isarangkool SN, Techawongstien S (2014) Effect of fruit position on fruit quality of ‘Num Dok Mai Sithong’ mango. Acta Hortic. SHS Acta Horticulturae 1111: XXIX International Horticultural Congress on Horticulture: Sustaining Lives, Livelihoods and Landscapes (IHC2014): IV International Symposium on Papaya, VIII International Pineapple Symposium, and International Symposium on Mango, pp 336–340Google Scholar
  16. Kayesh E, Lingfei S, Nicholas K, Xin S, Nadira B, Yanping Z, Jian H, Changnian S, Zong-M C, Jinggui F (2013) Fruit skin color and the role of anthocyanin. Acta Physiol Plant 35:2879–2890CrossRefGoogle Scholar
  17. Konczak I, Zhang W (2004) Anthocyanins: more than nature’s colors. J Biomed Biotechnol 5:239–240CrossRefGoogle Scholar
  18. Kondo S, Maeda M, Kobayashi S, Honda C (2002) Expression of anthocyanin biosynthetic genes in malus sylvestris L. ‘Mutsu’ non-red apples. J Hortic Sci Biotechnol 77:718–723CrossRefGoogle Scholar
  19. Lima AJ, Alvarenga AA, Marcelo RM, Deyse G, Elisa BL (2013) Chemical evaluation and effect of bagging new peach varieties introduced in southern Minas Gerais, Brazil. Food Sci Technol (Campinas) 33(3):434–440CrossRefGoogle Scholar
  20. Liu Y, Fei C, Lixin W, Rui M, Xiaojun Z, Zhengyang Z (2013) Fruit coloration and anthocyanin biosynthesis after bag removal in non-red and red apples (Malus× domestica Borkh). Molecules 18:1549–1563.  https://doi.org/10.3390/molecules18021549 CrossRefPubMedGoogle Scholar
  21. McGuire RG (1992) Reporting of objective color measurements. HortScience 27:1254–1255Google Scholar
  22. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29(9):e45.  https://doi.org/10.1093/nar/29.9.e45 CrossRefPubMedPubMedCentralGoogle Scholar
  23. Royal Horticulture Society (2007) Royal horticultural society color chart, 5th edn. Royal Horticultural Society, LondonGoogle Scholar
  24. Saure MC (1990) External control of anthocyanin formation in apple. Sci Hortic 42:181–218CrossRefGoogle Scholar
  25. Shivashankara KS, Jalikop SH, Roy TK (2010) Species variability for fruit antioxidant and radical scavenging abilities in mulberry. Int J Fruit Sci 10:355–366CrossRefGoogle Scholar
  26. Steyn WJ (2009) Prevalence and function of anthocyanins in fruits. In: Gould K, Davis K, Chris W (eds) Anthocyanins: biosynthesis, functions and applications. Springer Science Business Media, Berlin, pp 85–100Google Scholar
  27. Takos AM, Jaffe FW, Jacob SR, Bogs J, Robinson SP, Walker AR (2006) Light-induced expression of a MYB gene regulates anthocyanin biosynthesis in red apples. Plant Physiol 142(3):1216–1232CrossRefPubMedPubMedCentralGoogle Scholar
  28. Tyas JA, Hofman PJ, Underhill SJ, Bell KL (1998) Fruit canopy position and panicle bagging affects yield and quality of ‘Tai So’ lychee. Sci Hortic 72:203–213CrossRefGoogle Scholar
  29. Vasanthaiah HK, Kundapura VR, Kodthalu SS, Lalitha A, Pappana N, Gullara CM, Prasad TG (2006) Cloning and characterization of differentially expressed genes of internal breakdown in mango fruit (mangifera indica). J Plant Physiol 163:671–679CrossRefPubMedGoogle Scholar
  30. Voss DH (1992) Relating colorimeter measurement of plant color to the royal horticultural society colour chart. HortScience 27:1256–1260Google Scholar
  31. Wei YZ, Hu FC, Hu GB, Li XJ, Huang XM, Wang HC (2011) Differential expression of anthocyanin biosynthetic genes in relation to anthocyanin accumulation in the pericarp of litchi chinensis sonn. PLoS ONE 6(4):e19455CrossRefPubMedPubMedCentralGoogle Scholar
  32. Wu HX, Wang SB, Ma XW, Ma WH, Zhan RL, Yao QS (2013) Effect of bagging on fruit quality in mango. Acta Hortic 992:587–592CrossRefGoogle Scholar
  33. Xie R, Li Z, Shaolan H, Yongqiang Z, Shilai Y, Lie D (2011) Anthocyanin biosynthesis in fruit tree crops: genes and their regulation. Afr J Biotechnol 10(86):19890–19897Google Scholar

Copyright information

© Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature 2018

Authors and Affiliations

  • G. R. Karanjalker
    • 1
    • 2
  • K. V. Ravishankar
    • 1
  • K. S. Shivashankara
    • 1
  • M. R. Dinesh
    • 1
  1. 1.ICAR-Indian Institute of Horticultural Research (IIHR)BengaluruIndia
  2. 2.College of HorticultureUniversity of Horticultural Sciences, BagalkotBengaluruIndia

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