Polypeptide Alteration During The Cold Stratification In Mazzard Seed
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Many seeds of woody plants require low temperature for germination and that’s called seed dormancy has been investigated for relations of proteins by several researchers. We determined polypeptide profiles of cold-stratified Mazzard seeds. Seeds were studied in five different periods: seeds in dormancy (none stratified), 30, 60, 90 and 120 days cold-stratified. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE) of the soluble protein fractions from stratified and none stratified seeds was utilized for determination of changes in polypeptides. SDS-PAGE revealed a decrease in the concentrations of 14 polypeptides and appearance of four low-molecular weight (15.1, 16.3, 17.8 and 19.4 kDa) polypeptides were determined in 90 and 120 day stratified seeds. The study results exhibited the evidences of the involvement of protein changes in response of Mazzard seeds during cold stratification.
KeywordsMazzard Stratification Polypeptide Protein SDS-PAGE
Veränderung der Polypeptide während der Stratifikation von Saatgut der Vogelkirsche
SchlüsselwörterVogelkirsche Stratifikation Polypeptide Protein SDS-PAGE
This study was supported by TUBITAK (2209A), The Scientific and Technological Research Council of Turkey.
Conflict of interest
S. Aras, E. Arslan, A. Eşitken and M. Kılıç declare that they have no competing interests.
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