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Rational Rubber Extraction and Simultaneous Determination of Rubber Content and Molecular Weight Distribution in Taraxacum kok-saghyz Rodin by Size-Exclusion Chromatography

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Abstract

Taraxacum kok-saghyz Rodin (TKS) is a potential nature rubber source crop. To extract rubber from TKS effectively and quantitate the TKS rubber accurately, we developed a method that can simultaneously determine the rubber content and molecular weight distribution (MWD) of TKS by size-exclusion chromatography (SEC). The solvent types and rubber extraction schemes for sample pretreatment were discussed. The results indicated that the indirect ultrasonic method of extracting rubber from TKS roots required much shorter extraction time than conventional extraction method, and the scheme did not affect the characterization of MWD. In addition, SEC can be used for TKS rubber quantification with a limit of detection of 0.03 mg mL−1, and the intra-day and inter-day precision were 2.31% and 5.24%, respectively. The MWD of TKS rubber demonstrated a unimodal distribution. This developed method has been applied to the determination of real-life samples successfully.

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Acknowledgements

The authors acknowledge the support of the National Key Research and Development Program of China (2016YFF0203703), the NSFC Grant (51673012), and the Fundamental Research Funds for the Central Universities (PYBZ1828).

Funding

This study was funded by the National Key Research and Development Program of China (2016YFF0203703), the NSFC Grant (51673012), and the Fundamental Research Funds for the Central Universities (PYBZ1828).

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Authors and Affiliations

  1. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China

    Na Zhang, Xiang Ma, Jiahui Liu & Yiyang Dong

  2. Xinjiang Production and Construction Group Key Laboratory of Agricultural Products Processing, College of Life Science, Tarim University, Alar, 843300, Xinjiang, China

    Na Zhang

  3. Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Technology and Business University, Beijing, 100048, China

    Tianyang Guo

  4. Center of Advanced Elastomer Materials, College of Materials and Engineering, Beijing University of Chemical Technology, Beijing, 100029, China

    Jichuan Zhang

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  1. Na Zhang
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Correspondence to Yiyang Dong or Jichuan Zhang.

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Zhang, N., Guo, T., Ma, X. et al. Rational Rubber Extraction and Simultaneous Determination of Rubber Content and Molecular Weight Distribution in Taraxacum kok-saghyz Rodin by Size-Exclusion Chromatography. Chromatographia 82, 1459–1466 (2019). https://doi.org/10.1007/s10337-019-03773-2

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