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Simultaneous Determination of 20 Antibiotics in Bovine Colostrum Tablet Using UHPLC–MS/MS and SPE

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Abstract

A rapid and simple method was developed for the simultaneous determination of 20 antibiotics in bovine colostrum tablets using ultra-high-performance liquid chromatography–tandem mass spectrometry. The samples were prepared by a two-step extraction procedure followed by loading onto a reversed phase HLB cartridge for purification. The matrix-matched standard curves were used to compensate the matrix effects. Linearity was achieved for the analytes in the concentration ranges studied. The limits of detection (LODs) and limits of quantification (LOQs) for all samples were 0.05–2.03 and 0.16–6.78 μg kg−1, respectively. The mean recoveries were from 81.3 to 113.9% at three spiked levels, the relative standard deviations (RSDs, n = 6) ranged from 0.5 to 12.6%. The developed method has been successfully applied to detecting antibiotics in real samples; clindamycin, roxithromycin, oleandomycin and ofloxacin were detected.

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Acknowledgements

This work was supported by Science and technology project of General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China (no. 2016IK296); Youth science and technology talents fund project of Chongqing Municipal Science and Technology Commission (no. cstc2014kjrc-qnrc00002).

Funding

This study was supported by Science and technology project of General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China (no. 2016IK296); Youth science and technology talents fund project of Chongqing Municipal Science and Technology Commission (no. cstc2014kjrc-qnrc00002).

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Authors and Affiliations

  1. College of Pharmacy, Chongqing Medical University, Chongqing, 400016, China

    Jia Zheng & Zhaode Mu

  2. Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing, 400016, China

    Jia Zheng & Zhaode Mu

  3. Chongqing Entry-Exit Inspection and Quarantine Bureau, Chongqing, 400020, China

    Cunxian Xi, Guomin Wang, Shurui Cao & Bobin Tang

  4. Chongqing Engineering Technology Research Center of Import and Export Food Safety, Chongqing, 400020, China

    Cunxian Xi, Guomin Wang, Shurui Cao & Bobin Tang

Authors
  1. Jia Zheng
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  2. Cunxian Xi
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  3. Guomin Wang
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  4. Shurui Cao
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  5. Bobin Tang
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  6. Zhaode Mu
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Correspondence to Zhaode Mu.

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Zheng, J., Xi, C., Wang, G. et al. Simultaneous Determination of 20 Antibiotics in Bovine Colostrum Tablet Using UHPLC–MS/MS and SPE. Chromatographia 81, 947–957 (2018). https://doi.org/10.1007/s10337-018-3515-3

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  • DOI: https://doi.org/10.1007/s10337-018-3515-3

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