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Comparative Analysis of the Distribution of Copper in Green and Brown Brazilian Propolis Extracts

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Abstract

We proposed here a novel analytical procedure for copper speciation in green and brown propolis extracts using SEC—HPLC—GFAAS with 0.5% m v−1 SDS in 2.5 m mol L−1 Tris–HCl (pH 7.4) as the mobile phase buffer solution. Both basic (0.05 mol L−1 NaOH) and acid (0.05 mol L−1 HCl) conditions were evaluated for sample extraction. Depending on the extraction procedure, differences in copper distribution were identified. Copper was mainly associated with high-molecular-weight (HMW) fractions in green propolis extract when extracted with basic solution, whereas with acid extraction solution, only low-molecular-weight (LMW) fractions were obtained in both samples. Furthermore, combined analysis of results obtained using SEC-UV and GF AAS confirmed the association of copper with LMW and HMW species.

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Acknowledgements

The authors are grateful to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado do Pará (FAPESPA) for financial support. KGF, PON (Grant CNPq Nº 551983/2009-4) and JAN also thank the CNPq for providing research and fellowship support.

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Authors and Affiliations

  1. Grupo de Espectrometria Analítica Aplicada, Faculdade de Química, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Belém, PA, 66075-110, Brazil

    Patrícia O. Nunes, Fábio I. M. Carvalho & Kelly G. Fernandes Dantas

  2. Universidade Federal Rural da Amazônia, Campus Parauapebas, Parauapebas, PA, Brazil

    Fábio I. M. Carvalho

  3. Grupo de Análise Instrumental e Aplicada, Departamento de Química, Universidade Federal de São Carlos, São Carlos, SP, Brazil

    Joaquim A. Nóbrega

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  1. Patrícia O. Nunes
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  2. Fábio I. M. Carvalho
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  3. Joaquim A. Nóbrega
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  4. Kelly G. Fernandes Dantas
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Correspondence to Kelly G. Fernandes Dantas.

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Nunes, P.O., Carvalho, F.I.M., Nóbrega, J.A. et al. Comparative Analysis of the Distribution of Copper in Green and Brown Brazilian Propolis Extracts. Chromatographia 81, 623–629 (2018). https://doi.org/10.1007/s10337-018-3490-8

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  • DOI: https://doi.org/10.1007/s10337-018-3490-8

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