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Chromatographia

, Volume 81, Issue 4, pp 623–629 | Cite as

Comparative Analysis of the Distribution of Copper in Green and Brown Brazilian Propolis Extracts

  • Patrícia O. Nunes
  • Fábio I. M. Carvalho
  • Joaquim A. Nóbrega
  • Kelly G. Fernandes Dantas
Original

Abstract

We proposed here a novel analytical procedure for copper speciation in green and brown propolis extracts using SEC—HPLC—GFAAS with 0.5% m v−1 SDS in 2.5 m mol L−1 Tris–HCl (pH 7.4) as the mobile phase buffer solution. Both basic (0.05 mol L−1 NaOH) and acid (0.05 mol L−1 HCl) conditions were evaluated for sample extraction. Depending on the extraction procedure, differences in copper distribution were identified. Copper was mainly associated with high-molecular-weight (HMW) fractions in green propolis extract when extracted with basic solution, whereas with acid extraction solution, only low-molecular-weight (LMW) fractions were obtained in both samples. Furthermore, combined analysis of results obtained using SEC-UV and GF AAS confirmed the association of copper with LMW and HMW species.

Keywords

Propolis Fractionation Copper SEC on-line UV detection SEC off-line GF AAS 

Notes

Acknowledgements

The authors are grateful to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado do Pará (FAPESPA) for financial support. KGF, PON (Grant CNPq Nº 551983/2009-4) and JAN also thank the CNPq for providing research and fellowship support.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Cunha IBS, Sawaya ACHF, Caetano FM, Shimizu MT, Marcucci MC, Drezza FT, Povia GS, De O Carvalho P (2004) Factors that influence the yield and composition of Brazilian propolis extracts. J Braz Chem Soc 15:964–970CrossRefGoogle Scholar
  2. 2.
    Isla MI, Paredes-Guzman JF, Nieva-Moreno H, Koo MI, Park YK (2005) Some chemical composition and biological activity of northern Argentine propolis. J Agric Food Chem 53:1166–1172CrossRefGoogle Scholar
  3. 3.
    Sforcin JM, Orsi RO, Bankova V (2005) Effect of propolis, some isolated compounds and its source plant on antibody production. J Ethnopharm 98:301–305CrossRefGoogle Scholar
  4. 4.
    Marcucci MC (1996) Propriedades biológicas e terapêuticas dos constituintes químicos da própolis. Quim Nova 19:529–536Google Scholar
  5. 5.
    Park YK, Ikegaki M, Da Silva Abreu JA, Freire Alcici NM (1998) Estudo da preparação dos extratos de própolis e suas aplicações. Ciênc Tecnol Aliment 18:313–318CrossRefGoogle Scholar
  6. 6.
    Park YK, Ikegaki M (1998) Preparation of water and ethanolic extracts of propolis and evaluation of the preparation. Biosci Biotechnol Biochem 62:2230–2232CrossRefGoogle Scholar
  7. 7.
    Pereira AS, Silva Seixas FRM, Aquino Neto FR (2002) Própolis: 100 anos de pesquisa e suas perspectivas futuras. Quím Nova 25:321–326CrossRefGoogle Scholar
  8. 8.
    Banskota AH, Tezuka Y, Kadota S (2001) Recent progress in pharmacological research of propolis. Phytother Res 15:561–571CrossRefGoogle Scholar
  9. 9.
    Bankova VS, Castro SL, Marcucci MC (2000) Propolis: recent advances in chemistry and plant origin. Apidologie 31:3–15CrossRefGoogle Scholar
  10. 10.
    Sanz-Medel A (2005) From metalloproteomics to heteroatom-tagged proteomics. Anal Bioanal Chem 381:1–2CrossRefGoogle Scholar
  11. 11.
    Cornelis R, Caruso J, Crews H, Heumann K (2003) Handbook of elemental speciation: techniques and methodology. Wiley, ChichesterCrossRefGoogle Scholar
  12. 12.
    Veiga Junior VF, Pinto AC, Maciel MAM (2005) Plantas medicinais: cura segura. Quim Nova 28:519–528CrossRefGoogle Scholar
  13. 13.
    Negri G, Salatino MLF, Salatino A (2003) “Green propolis”: unreported constituents and a movel compound from chloroform extracts. J Apic Res 42:39–41CrossRefGoogle Scholar
  14. 14.
    Araújo CL, Bezerra IWL, Dantas IC, Lima TVS, Oliveira AS, Miranda MRA, Leite EL, Sales MP (2007) Biological activity of proteins from pulps of tropical fruits. Food Chem 85:107–110CrossRefGoogle Scholar
  15. 15.
    Naozuka J, Oliveira PV (2007) Cu, Fe, Mn and Zn distribution in protein fractions of Brazil-nut, cupuassu seed and coconut pulp by solid-liquid extraction and electrothermal atomic absorption spectrometry. J Braz Chem Soc 18:1547–1553CrossRefGoogle Scholar
  16. 16.
    Ponce de León CA, Montes-Bayón M, Caruso JA (2002) Elemental speciation by chromatographic separation with inductively coupled plasma mass spectrometry detection. J Chromatogr A 974:1–21CrossRefGoogle Scholar
  17. 17.
    Welz B, Sperling M (1999) Atomic absorption spectrometry. Weinheim, GermanyGoogle Scholar
  18. 18.
    Butcher DJ, Sneddon J (1998) A practical guide to graphite furnace atomic absorption spectrometry. Wiley, New YorkGoogle Scholar
  19. 19.
    Naozuka J, Marana SR, Oliveira PV (2010) Water-soluble Cu, Fe, Mn and Zn species in nuts and seeds. J Food Comp Anal 23:78–85CrossRefGoogle Scholar
  20. 20.
    Soldado AB, Blanco González E, Sanz-Medel A (1997) Quantitative studies of aluminium binding species in human uremic serum by fast protein liquid chromatography coupled with electrothermal atomic absorption spectrometry. Analyst 122:573–577CrossRefGoogle Scholar
  21. 21.
    Kannankumarath SS, Wrobel K, Wuilloud RG (2005) Studying the distribution pattern of selenium in nut proteins with information obtained from SEC-UV-ICP-MS and CE-ICP-MS. Talanta 66:153–159CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Patrícia O. Nunes
    • 1
  • Fábio I. M. Carvalho
    • 1
    • 2
  • Joaquim A. Nóbrega
    • 3
  • Kelly G. Fernandes Dantas
    • 1
  1. 1.Grupo de Espectrometria Analítica Aplicada, Faculdade de Química, Instituto de Ciências Exatas e NaturaisUniversidade Federal do ParáBelémBrazil
  2. 2.Universidade Federal Rural da AmazôniaParauapebasBrazil
  3. 3.Grupo de Análise Instrumental e Aplicada, Departamento de QuímicaUniversidade Federal de São CarlosSão CarlosBrazil

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