, Volume 81, Issue 4, pp 699–706 | Cite as

Development and Validation of an HPLC Method for the Simultaneous Determination of Flurbiprofen and Chlorhexidine Gluconate

  • Beril Deniz Işık
  • Ebru Türköz Acar
Short Communication


Flurbiprofen (FBP) is a strong nonsteroidal anti-inflammatory drug which has analgesic, antipyretic and anti-inflammatory effects. Chlorhexidine gluconate (CHG) is a potent antibacterial agent. Nowadays, FBP and CHG as a combination are used in some pharmaceutical products. In this study, an analysis method was developed for the simultaneous determination of FBP and CHG using high performance liquid chromatography (HPLC) technique. During analysis, Agilent Poroshell 120 EC-C18 column was used as stationary phase with a guard column having the same properties. Sodium phosphate buffer solution (100 mM, pH 2.5) and acetonitrile were used as mobile phase. The chromatography was performed by gradient elution at a flow rate of 0.5 mL min−1. Analytes were detected at 248 nm using a diode array detector (DAD). The developed method was validated according to United States Pharmacopoeia. It was found to be linear in the concentration range between 1 and 25 ppm. Determination coefficient was 0.9999 for FBP and CHG. The developed method was applied successfully for HPLC analysis of commercial pharmaceutical products including FBP and CHG.

Graphical abstract


HPLC Flurbiprofen Chlorhexidine gluconate Analysis Validation 



This work was financially supported by the Yeditepe University, İstanbul, Turkey.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10337_2018_3485_MOESM1_ESM.docx (130 kb)
Supplementary material 1 (DOCX 130 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Pharmaceutical Chemistry, Institute of Health SciencesYeditepe UniversityIstanbulTurkey
  2. 2.Analytical Chemistry, Faculty of PharmacyYeditepe UniversityIstanbulTurkey

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