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Chromatographia

, Volume 81, Issue 2, pp 239–245 | Cite as

HPLC Characterization of the Association Mechanism of Organic Compounds with β Cyclodextrin Using a Novel Boron Nitride Nanotube Particulate Stationary Phase

  • Yves Claude Guillaume
  • Lydie Lethier
  • Claire Andre
Original
  • 83 Downloads

Abstract

In this paper, a simple homogeneous coating of silica spherical particles with pristine boron nitride nanotubes (BNNTs) was described. BNNTs dissolved in dimethylacetamide (DMAc) were mixed with amino-functionalized silica particles having a 5 μm diameter. Favorable interaction between the amino group and the BNNT surfaces induces the absorption of the BNNTs on the silica. The BNNT-coated silica particles were used as stationary phase for HPLC. For the first time, it was demonstrated that this new particulate BNNT stationary phase can be used for the study of the complexation of solute molecules (terpene molecules used as test drugs in this work) with β cyclodextrin (βCD). The apparent formation constants Kf of terpene derivative/βCD were in the same magnitude as those reported in the literature. The plot of Kf versus the water fraction in the methanol/water mobile phase showed that the BNNT surface played an active role in the complex formation due to terpene/BNNT-specific polar interactions. This work demonstrated that our novel particulate BNNT HPLC stationary phase was an efficient tool to study molecular recognition mechanism and more specifically the association between a drug substance and a target molecule with the aim of reaching biopharmaceutic and clinical applications.

Keywords

HPLC Boron nitride nanotubes Silica particle βCD Methanol Association constant 

Notes

Compliance with ethical standards

Conflict of interest

The three authors declare that there is no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Yves Claude Guillaume
    • 1
    • 2
    • 3
  • Lydie Lethier
    • 1
    • 2
    • 3
  • Claire Andre
    • 1
    • 2
    • 3
  1. 1.Univ Franche-ComtéBesanconFrance
  2. 2.CHRU BesançonBesanconFrance
  3. 3.Pôle Chimie Analytique Bioanalytique et Physique (PCABP) EA481 Neurosciences Intégratives et CliniquesBesanconFrance

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