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γ-Fe2O3 Nanoparticles Covered with Glutathione-Modified Quantum Dots as a Fluorescent Nanotransporter

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Abstract

The present paper describes the synthesis, characterization, and utilization of multi-functional magnetic conjugates that integrate optical and magnetic properties in a single structure for use in many biomedical applications. Spontaneous interaction with eukaryotic cell membrane (HEK-239 cell culture) was determined using fluorescence microscopy, and fluorescence analyses. Both, differences in excitation, and emission wavelength were observed, caused by glutathione intake by cells, resulting in disintegration of core–shell structure of quantum dots, as well as adhesion of conjugate onto cell surface. When compared with quantum dots fluorescent properties, HEK-239 cells with incorporated nanoconjugate exhibited two excitation maxima (λ ex = 430 and 390 nm). Simultaneously, application of ideal λ ex for quantum dots (λ ex = 430 nm), resulted in two emission maxima (λ = 740 and 750 nm). This nanoconjugate fulfills the requirements of term theranostics, because it can be further functionalized with biomolecules as DNA, proteins, peptides or antibodies, and thus serves as a tool for therapy in combination with simultaneous treatment.

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Acknowledgments

The authors are grateful to CEITEC CZ.1.05/1.1.00/02.0068 for financial support. The authors also with to express their thanks to Lukas Melichar for perfect technical assistance.

Conflict of interest

The authors have declared no conflict of interest.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic

    Zbynek Heger, Natalia Cernei, Iva Blazkova, Pavel Kopel, Ondrej Zitka, Vojtech Adam & Rene Kizek

  2. Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, 616 00, Brno, Czech Republic

    Natalia Cernei, Iva Blazkova, Pavel Kopel, Michal Masarik, Ondrej Zitka, Vojtech Adam & Rene Kizek

  3. Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic

    Michal Masarik

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  1. Zbynek Heger
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  2. Natalia Cernei
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  3. Iva Blazkova
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Correspondence to Rene Kizek.

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Heger, Z., Cernei, N., Blazkova, I. et al. γ-Fe2O3 Nanoparticles Covered with Glutathione-Modified Quantum Dots as a Fluorescent Nanotransporter. Chromatographia 77, 1415–1423 (2014). https://doi.org/10.1007/s10337-014-2732-7

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  • DOI: https://doi.org/10.1007/s10337-014-2732-7

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