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Capillary Electrophoresis Chemiluminescence for the Analysis of Flavonoids in Pharmaceuticals and Human Plasma

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Abstract

A capillary electrophoresis (CE)-chemiluminescence (CL) method has been developed for the determination of the pharmacologically active flavonoids, including rutin and quercetin in pharmaceuticals and human plasma containing Ginkgo biloba leaves extract (EGb). The separation was conducted in borate buffer and luminol. The post-column CL reagent was K3Fe(CN)6 in NaOH medium. Rutin and quercetin were baseline separated within 10 min with detection limits of 1.0 and 5.0 nM, respectively. The maximum intra- and inter-day relative standard deviations (RSD) of migration time of analytes were <3.5 %. Moreover, the high selectivity of the CL detection and the high-separation efficiency of CE render the method the potential of quick analyzing the pharmacologically active substances in complex matrix with satisfactory results.

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Acknowledgments

Financial support from the National Natural Science Foundation of China (20445002) and the Natural Science Foundation of Shanxi Province (20051022) is gratefully acknowledged.

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Authors and Affiliations

  1. School of Chemistry and Material Science, Shanxi Normal University, Linfen, 041004, Shanxi, People’s Republic of China

    Jia Wang & Suqin Han

  2. School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, People’s Republic of China

    Jia Wang

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  1. Jia Wang
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  2. Suqin Han
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Correspondence to Suqin Han.

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Wang, J., Han, S. Capillary Electrophoresis Chemiluminescence for the Analysis of Flavonoids in Pharmaceuticals and Human Plasma. Chromatographia 76, 715–718 (2013). https://doi.org/10.1007/s10337-013-2439-1

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  • DOI: https://doi.org/10.1007/s10337-013-2439-1

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