Phylogenetic analysis of the pathogen causing eggplant brown leaf spot

Abstract

Eggplant brown leaf spot caused by Rhizoctonia solani AG-3 was first found in Japan in 1976, but its subgroup remained undetermined for over 40 years. We conducted a molecular phylogenetic analysis using three loci (rDNA-ITS, rDNA-IGS1, and tef-1α) of two Iwate isolates, newly obtained in this study, and one Tokyo isolate, which was previously identified as AG-2-2 IV and reported as an additional pathogen causing this disease. The phylogenetic tree based on these three loci revealed that the three isolates belonged to AG-3 TM. Eggplant is the first non-tomato host of AG-3 TM to be identified in Japan.

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Correspondence to Tomoo Misawa.

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The nucleotide sequence data reported are available in the DDBJ/EMBL/ GenBank databases as accessions LC533879–533887.

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Misawa, T., Iwadate, Y. & Kurose, D. Phylogenetic analysis of the pathogen causing eggplant brown leaf spot. J Gen Plant Pathol 87, 123–126 (2021). https://doi.org/10.1007/s10327-021-00982-2

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Keywords

  • Brown leaf spot
  • Eggplant
  • Rhizoctonia solani AG-3 TM
  • Solanaceae