Specific detection and quantification of Ralstonia pseudosolanacearum race 4 strains from Zingiberaceae plant cultivation soil by MPN-PCR

Abstract

The most probable number-polymerase chain reaction (MPN-PCR) method was assessed for the specific detection and quantification of Ralstonia pseudosolanacearum race 4 strains in soil from Zingiberaceae fields in Japan. Based on the genome sequence analysis of two bacterial strains [MAFF 211479 (type I) and MAFF 211472 (type II)] isolated from ginger, race 4 type I- and type II-specific nested PCR primer sets were designed, respectively. The MPN-PCR using these primer sets efficiently detected and quantified the pathogen in naturally and artificially infested soils.

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Acknowledgements

We thank Dr. Y. Inoue (National Agricultural Research Center, NARO) for technical advice on the MPN-PCR test and Dr. K. Nakaho (Institute of Vegetable and Floriculture Science, NARO), Dr. K. Yano, Mr. Y. Morita (Kochi Agricultural Research Center), Dr. S. Kawano, Ms. M. Yamashiro, Mr. A. Ajitomi, and Dr. A. Ooshiro (Okinawa Agricultural Research Center) for technical support in preparing materials (bacterial strains, plant materials, and/or field soil). This work was supported by the grant from the Project of the NARO Bio-Oriented Technology Research Advancement Institution (Research Program on Development of Innovative Technology no. 29014C).

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Correspondence to Mitsuo Horita.

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The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession numbers LC508241 and LC508242.

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Horita, M., Sakai, Y.K. Specific detection and quantification of Ralstonia pseudosolanacearum race 4 strains from Zingiberaceae plant cultivation soil by MPN-PCR. J Gen Plant Pathol (2020). https://doi.org/10.1007/s10327-020-00939-x

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Keywords

  • Ralstonia pseudosolanacearum
  • Race 4
  • Zingiberaceae
  • MPN-PCR
  • Soil
  • Quantification