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Ampeloviruses associated with incomplete flower syndrome and leaf-edge necrosis in Japanese apricot

  • Viral and Viroid Diseases
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

Japanese apricot (Prunus mume Sieb. et Zucc.) trees produce popular fruits and are also used in Japan as ornamental flowering trees. Since the 1980s, graft-transmissible symptoms (e.g., incomplete flowers and leaf-edge necrosis) have been observed on ‘Nanko’ trees in Wakayama Prefecture. We here describe the detection of viruses associated with these symptoms in Japanese apricot trees using DECS analysis (i.e., double-stranded RNA [dsRNA] isolation, exhaustive amplification, cloning, and sequencing). We isolated dsRNA from symptomatic flowers, including dsRNA of plum bark necrosis stem pitting-associated virus (PBNSPaV), little cherry virus 2 (LChV-2), and a Luteovirus-like sequence. The reverse transcription polymerase chain reaction results suggested that PBNSPaV and LChV-2 may induce the development of incomplete flowers and leaf-edge necrosis. In contrast, the Luteovirus-like sequence is apparently unrelated to these symptoms. Additional investigations are necessary to clarify the relationship between the symptoms and viral infections.

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Acknowledgements

The authors thank the growers for their cooperation and providing access to trees used in this study. They also thank Mr. Y. Tanimoto, Mr. Y. Akagi and Dr. Y. Tsuchida (Wakayama Fruit Tree Experiment Station) for helpful comments and suggestions on the study, and Dr. Y. Tomitaka (Central Region Agricultural Research Center, NARO) for providing the CMV sample. This study was funded by the NARO.

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Authors and Affiliations

  1. Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), 2-1 Fujimoto, Tsukuba, Ibaraki, 305-8605, Japan

    Ryoji Nakaune, Eiko Nakazono-Nagaoka & Takashi Fujikawa

  2. Japanese Apricot Laboratory, Wakayama Fruit Tree Experiment Station, 1416-7 Higashi-Honjo, Minabe, Wakayama, 645-0021, Japan

    Tomoaki Takeda & Koji Numaguchi

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  1. Ryoji Nakaune
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  2. Tomoaki Takeda
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  3. Koji Numaguchi
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  4. Eiko Nakazono-Nagaoka
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  5. Takashi Fujikawa
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Correspondence to Ryoji Nakaune.

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Nakaune, R., Takeda, T., Numaguchi, K. et al. Ampeloviruses associated with incomplete flower syndrome and leaf-edge necrosis in Japanese apricot. J Gen Plant Pathol 84, 202–207 (2018). https://doi.org/10.1007/s10327-018-0781-8

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  • DOI: https://doi.org/10.1007/s10327-018-0781-8

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