Abstract
Leaf samples of Cucurbita pepo with yellow mosaic disease symptoms were collected in 2012. Rolling circle amplification and PCR amplification with begomovirus-specific primers confirmed the presence of an Old World bipartite begomovirus, an alphasatellite and a betasatellite. Molecular analysis of full-length sequences showed that Tomato leaf curl New Delhi virus (DNA-A) is associated with its cognate DNA-B, Papaya leaf curl betasatellite and a novel alphasatellite. To the best of our knowledge, this is the first report of an alphasatellite and a betasatellite associated with a bipartite begomovirus.
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Acknowledgements
This article does not contain any studies with human participants or animals performed by the author. SA is supported by a scholarship from the National University of Sciences and Technology (NUST), Islamabad, endowment fund. Thanks to Muhammad Ali (ASAB, NUST) for technical assistance.
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Anwar, S. Distinct association of an alphasatellite and a betasatellite with Tomato leaf curl New Delhi virus in field-infected cucurbit. J Gen Plant Pathol 83, 185–188 (2017). https://doi.org/10.1007/s10327-017-0709-8
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DOI: https://doi.org/10.1007/s10327-017-0709-8