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First report of ‘Candidatus Phytoplasma asteris’ infecting hydrangea showing phyllody in Japan

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Abstract

Phytoplasma-induced floral malformations such as virescence, phyllody, and proliferation were observed on hydrangeas in Gunma Prefecture, Japan. Phylogenetic analyses based on 16S rRNA, secY, groEL, and amp gene sequences indicated that the affected hydrangea plants were associated with phytoplasmas belonging to ‘Candidatus Phytoplasma asteris’, but not to ‘Ca. P. japonicum’, which occurs in hydrangeas showing phyllody in Japan. This is the first molecular evidence of an association of ‘Ca. P. asteris’ with hydrangea plants in Japan.

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References

  • Bertaccini A, Davis RE, Hammond RW, Vibio M, Bellardi MG, Lee IM (1992) Sensitive detection of mycoplasmalike organisms in field-collected and in vitro propagated plants of Brassica, Hydrangea and Chrysanthemum by polymerase chain-reaction. Ann Appl Biol 121:593–599

    Article  Google Scholar 

  • Cousin MT, Sharma AK (1986) Association of mycoplasmalike organisms (MLOs) with mild type of Hydrangea virescence: a study with 60–1000 nm thick sections. J Phytopathol 115:274–282

    Article  Google Scholar 

  • Firrao G, Andersen M, Bertaccini A, Boudon E, Bové JM, Daire X, Davis RE et al (2004) ‘Candidatus Phytoplasma’, a taxon for the wall-less, non-helical prokaryotes that colonize plant phloem and insects. Int J Syst Evol Microbiol 54:1243–1255

    Article  CAS  Google Scholar 

  • Hiruki C, Rong XD, Deng SJ (1994) Hydrangea virescence, a disease associated with mycoplasmalike organism in Canada. Acta Hort 377:325–333

    Google Scholar 

  • Jung HY, Sawayanagi T, Kakizawa S, Nishigawa H, Wei W, Oshima K, Miyata S, Ugaki M, Hibi T, Namba S (2003) ‘Candidatus Phytoplasma ziziphi’, a novel phytoplasma taxon associated with jujube witches’-broom disease. Int J Syst Evol Microbiol 53:1037–1041

    Article  PubMed  CAS  Google Scholar 

  • Kakizawa S, Oshima K, Jung HY, Suzuki S, Nishigawa H, Arashida R, Miyata S, Ugaki M, Kishino H, Namba S (2006) Positive selection acting on a surface membrane protein of the plant-pathogenic phytoplasmas. J Bacteriol 188:3424–3428

    Article  PubMed  CAS  Google Scholar 

  • Kanehira T, Hirokoshi N, Yamakita Y, Shinohara M (1996) Occurrence of hydrangea phyllody in Japan and detection of the causal phytoplasma. Ann Phytopathol Soc Japan 62:537–540

    Article  Google Scholar 

  • Kim YH (1980) Studies on mycoplasma-like organisms associated with witches’ broom of Rhus javanica I. J Korean Forest Soc 47:1–15

    Google Scholar 

  • Kim YH, So IY, Chai JK, Cheong SS (1990) Studies on mycoplasma-like organism associated with witches’ broom of Rhus javanica II. Transmission by insect vector and dodder. Korean J Plant Pathol 6:119–124

    Google Scholar 

  • Kirkpatrick BC (1992) Mycoplasma-like organisms: plant and invertebrate pathogens. In: Balows A et al (eds) The prokaryotes, 2nd edn. Springer, New York, pp 4050–4067

    Google Scholar 

  • Kusunoki M, Miyashita S, Shiomi T, Ohashi A, Nohira T (1997) Leaf-hopper (Hishimonus sellatus) transmission of phytoplasmas of rhus yellows and jujube witches’ broom in Japan (Abstract in Japanese). Ann Phytopathol Soc Japan 63:257

    Google Scholar 

  • Kusunoki M, Shiomi T, Kobayashi M, Okudaira T, Ohashi A, Nohira T (2002) A leafhopper (Hishimonus sellatus) transmits phylogenetically distant phytoplasmas: rhus yellows and hovenia witches’ broom phytoplasma. J Gen Plant Pathol 68:147–154

    Article  CAS  Google Scholar 

  • Lee IM, Zhao Y, Bottner KD (2006) SecY gene sequence analysis for finer differentiation of diverse strains in the aster yellows phytoplasma group. Mol Cell Probes 20:87–91

    Article  PubMed  CAS  Google Scholar 

  • McCoy RE, Caudwell A, Chang CJ, Chen TA, Chiykowski LN, Cousin MT, Dale JL et al (1989) Plant diseases associated with mycoplasma-like organisms. In: Whitcomb RF, Tully JG (eds) The mycoplasmas, vol 5. Academic Press, San Diego, pp 545–640

    Chapter  Google Scholar 

  • Mitrović J, Kakizawa S, Duduk B, Oshima K, Namba S, Bertaccini A (2011) The groEL gene as an additional marker for finer differentiation of ‘Candidatus Phytoplasma asteris’-related strains. Ann Appl Biol 159:41–48

    Article  Google Scholar 

  • Sawayanagi T, Horikoshi N, Kanehira T, Shinohara M, Bertaccini A, Cousin MT, Hiruki C, Namba S (1999) ‘Candidatus Phytoplasma japonicum’, a new phytoplasma taxon associated with Japanese Hydrangea phyllody. Int J Syst Bacteriol 49:1275–1285

    Article  PubMed  CAS  Google Scholar 

  • Schneider B, Seemüller E (1994a) Presence of two sets of ribosomal genes in phytopathogenic mollicutes. Appl Environ Microbiol 60:3409–3412

    PubMed  CAS  Google Scholar 

  • Schneider B, Seemüller E (1994b) Studies on taxonomic relationships of mycoplasma-like organisms by southern blot analysis. J Phytopathol 141:173–185

    Article  Google Scholar 

  • Sugawara K, Himeno M, Keima T, Kitazawa Y, Maejima K, Oshima K, Namba S (2012) Rapid and reliable detection of phytoplasma by loop-mediated isothermal amplification targeting a housekeeping gene. J Gen Plant Pathol 78:389–397

    Article  CAS  Google Scholar 

  • Suzuki S, Oshima K, Kakizawa S, Arashida R, Jung HY, Yamaji Y, Nishigawa H, Ugaki M, Namba S (2006) Interaction between the membrane protein of a pathogen and insect microfilament complex determines insect-vector specificity. Proc Natl Acad Sci USA 103:4252–4257

    Article  PubMed  CAS  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work was supported by Grants-in-Aids for Scientific Research from the Japan Society for the Promotion of Science, and by the Funding Program for Next Generation World-Leading Researchers (Project: GS005).

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Correspondence to Shigetou Namba.

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Takinami, Y., Maejima, K., Takahashi, A. et al. First report of ‘Candidatus Phytoplasma asteris’ infecting hydrangea showing phyllody in Japan. J Gen Plant Pathol 79, 209–213 (2013). https://doi.org/10.1007/s10327-013-0445-7

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  • DOI: https://doi.org/10.1007/s10327-013-0445-7

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