Abstract
Saccharomyces cerevisiae strains with favorable characteristics are preferred for application in industries. However, the current ability to reprogram a yeast cell on the genome scale is limited due to the complexity of yeast ploids. In this study, a method named genome replication engineering-assisted continuous evolution (GREACE) was proved efficient in engineering S. cerevisiae with different ploids. Through iterative cycles of culture coupled with selection, GREACE could continuously improve the target traits of yeast by accumulating beneficial genetic modification in genome. The application of GREACE greatly improved the tolerance of yeast against acetic acid compared with their parent strain. This method could also be employed to improve yeast aroma profile and the phenotype could be stably inherited to the offspring. Therefore, GREACE method was efficient in S. cerevisiae engineering and it could be further used to evolve yeast with other specific characteristics.
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Funding
This study was financially supported by the National Science Foundation (nos. 31571942, and 31601558), the National High Technology Research and Development Program 863 (no. 2013AA102106-03), Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Program of Introducing Talents of Discipline to Universities (no. 111-2-06), and the Fundamental Research Funds for the Central Universities (JUSRP51306A, JUSRP51402A and JUDCF13008).
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Xu, X., Liu, C., Niu, C. et al. Rationally designed perturbation factor drives evolution in Saccharomyces cerevisiae for industrial application. J Ind Microbiol Biotechnol 45, 869–880 (2018). https://doi.org/10.1007/s10295-018-2057-x
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DOI: https://doi.org/10.1007/s10295-018-2057-x