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Proteins differentially expressed during limonene biotransformation by Penicillium digitatum DSM 62840 were examined using iTRAQ labeling coupled with 2D-LC–MS/MS

  • Genetics and Molecular Biology of Industrial Organisms - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

This study focused on the differences in protein expression at various periods during limonene biotransformation by Penicillium digitatum DSM 62840. A total of 3644 protein-species were quantified by iTRAQ during limonene biotransformation (0 and 12 h). A total of 643 proteins were differentially expressed, 316 proteins were significantly up-regulated and 327 proteins were markedly down-regulated. GO, COG, and pathway enrichment analysis showed that the differentially expressed proteins possessed catalytic and binding functions and were involved in a variety of cellular and metabolic process. Furthermore, the enzymes involved in limonene transformation might be related to cytochrome P-450. This study provided a powerful platform for further exploration of biotransformation, and the identified proteins provided insight into the mechanism of limonene transformation.

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Acknowledgments

This work was supported by Major Scientific and Technological Innovation Project in Hubei Province (2015ABA035), National Natural Science Foundation of China (Program No. 31101239) and the Fundamental Research Funds for the Central Universities (Program No. 2013PY097).

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Authors and Affiliations

  1. Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Lu-Lu Zhang, Yan Zhang, Jing-Nan Ren, Yan-Long Liu, Jia-Jia Li, Ya-Nan Tai, Shu-Zhen Yang, Si-Yi Pan & Gang Fan

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  1. Lu-Lu Zhang
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Correspondence to Gang Fan.

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Zhang, LL., Zhang, Y., Ren, JN. et al. Proteins differentially expressed during limonene biotransformation by Penicillium digitatum DSM 62840 were examined using iTRAQ labeling coupled with 2D-LC–MS/MS. J Ind Microbiol Biotechnol 43, 1481–1495 (2016). https://doi.org/10.1007/s10295-016-1826-7

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