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Efficient bioconversion of l-glutamate to γ-aminobutyric acid by Lactobacillus brevis resting cells

  • Biocatalysis - Review
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

This work investigated the efficient bioconversion process of l-glutamate to GABA by Lactobacillus brevis TCCC 13007 resting cells. The optimal bioconversion system was composed of 50 g/L 48 h cultivated wet resting cells, 0.1 mM pyridoxal phosphate in glutamate-containing 0.6 M citrate buffer (pH 4.5) and performed at 45 °C and 180 rpm. By 10 h bioconversion at the ratio of 80 g/L l-glutamic acid to 240 g/L monosodium glutamate, the final titer of GABA reached 201.18 g/L at the molar bioconversion ratio of 99.4 %. This process presents a potential for industrial and commercial applications and also offers a promising feasibility of continuous GABA production coupled with fermentation. Besides, the built kinetics model revealed that the optimum operating conditions were 45 °C and pH 4.5, and the bioconversion kinetics at low ranges of substrate concentration (0 < S < 80 g/L) was assumed to follow the classical Michaelis–Menten equation.

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Acknowledgments

This study was supported by the National Basic Research Program of China (973 Program) (2013CB734004), the National High-tech R&D Program (863 Program) (2012AA021032), and the National Natural Science Foundation of China (31370075 & 31471725).

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Authors and Affiliations

  1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Xiufeng Shi, Chuanyou Chang, Shenxi Ma, Yibing Cheng, Jun Zhang & Qiang Gao

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  1. Xiufeng Shi
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  2. Chuanyou Chang
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  3. Shenxi Ma
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  4. Yibing Cheng
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  5. Jun Zhang
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  6. Qiang Gao
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Correspondence to Qiang Gao.

Additional information

This article is dedicated to Dr. Arnold L. Demain for his 90th birthday, and his lifetime devotion and worldwide impact on industrial microbiology and biotechnology.

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Shi, X., Chang, C., Ma, S. et al. Efficient bioconversion of l-glutamate to γ-aminobutyric acid by Lactobacillus brevis resting cells. J Ind Microbiol Biotechnol 44, 697–704 (2017). https://doi.org/10.1007/s10295-016-1777-z

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  • DOI: https://doi.org/10.1007/s10295-016-1777-z

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