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Different effects of Porphyromonas gingivalis lipopolysaccharide and TLR2 agonist Pam3CSK4 on the adhesion molecules expression in endothelial cells

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Abstract

Recent researches suggest an association between periodontitis and cardiovascular disease. Periodontopathic bacteria and/or their component might play a role in the development of atherosclerotic lesions. In the present study, we investigated in vitro the effect of Porphyromonas gingivalis lipopolysaccharide (LPS) on the expression of adhesion molecules in human umbilical vein endothelial cells (HUVECs) as well as its effect on Escherichia coli LPS-induced response. The effect of P. gingivalis LPS was compared with that of toll-like receptor 2 agonist synthetic triacylated lipoprotein Pam3-Cys-Ser-(Lys)4 (Pam3CSK4). Gene and protein expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and E-selectin were measured using RT-PCR and flow cytometry, respectively. P. gingivalis LPS stimulated the expression levels of all adhesion molecules in a dose-dependent manner. However, the response of HUVECs to P. gingivalis LPS was markedly lower than that to E. coli LPS. Moreover, P. gingivalis LPS attenuated E. coli LPS-induced responses when HUVECs were simultaneously stimulated with both kinds of LPS. Treatment with Pam3CSK4 resulted in a minor increase of adhesion molecule expression and did not diminish E. coli LPS-induced responses. Our data suggest that P. gingivalis LPS induces in vitro the expression of adhesion molecules in endothelial cells, which might promote atherogenesis. Qualitatively different responses of HUVECs to P. gingivalis LPS and Pam3CSK4 suggest that besides TLR2 other signaling pathways might be involved in the effects of P. gingivalis LPS.

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Acknowledgments

The study was supported by author’s institution (Bernhard Gottlieb School of Dentistry). The authors thank Mrs. Phuong Quynh Nguyen and Mrs. Hedwig Rutschek for their help in performing the experiments.

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Division of Oral Biology, Bernhard Gottlieb School of Dentistry, Medical University of Vienna, Sensengasse2a, 1090, Vienna, Austria

    Oleh Andrukhov & Xiaohui Rausch-Fan

  2. Division of Conservative Dentistry and Periodontology, Bernhard Gottlieb School of Dentistry, Medical University of Vienna, Sensengasse2a, 1090, Vienna, Austria

    Ilse Steiner & Andreas Moritz

  3. Yantai Stomatological Hospital, Binzhou Medical University, 32 Qingquan RD, Yantai, 264001, China

    Shutai Liu

  4. Division of Orthodontics, Bernhard Gottlieb School of Dentistry, Medical University of Vienna, Sensengasse2a, 1090, Vienna, Austria

    Hans Peter Bantleon & Xiaohui Rausch-Fan

Authors
  1. Oleh Andrukhov
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  2. Ilse Steiner
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  3. Shutai Liu
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  4. Hans Peter Bantleon
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  5. Andreas Moritz
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  6. Xiaohui Rausch-Fan
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Corresponding authors

Correspondence to Oleh Andrukhov or Xiaohui Rausch-Fan.

Additional information

O. Andrukhov and I. Steiner contributed equally.

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Andrukhov, O., Steiner, I., Liu, S. et al. Different effects of Porphyromonas gingivalis lipopolysaccharide and TLR2 agonist Pam3CSK4 on the adhesion molecules expression in endothelial cells. Odontology 103, 19–26 (2015). https://doi.org/10.1007/s10266-013-0146-x

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  • DOI: https://doi.org/10.1007/s10266-013-0146-x

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