Journal of Plant Research

, Volume 131, Issue 1, pp 99–110 | Cite as

Physiological responses and antioxidant enzyme changes in Sulla coronaria inoculated by cadmium resistant bacteria

  • Manel Chiboub
  • Salwa Harzalli Jebara
  • Omar Saadani
  • Imen Challougui Fatnassi
  • Souhir Abdelkerim
  • Moez Jebara
Regular Paper


Plant growth promoting bacteria (PGPB) may help to reduce the toxicity of heavy metals on plants growing in polluted soils. In this work, Sulla coronaria inoculated with four Cd resistant bacteria (two Pseudomonas spp. and two Rhizobium sullae) were cultivated in hydroponic conditions treated by Cd; long time treatment 50 µM CdCl2 for 30 days and short time treatment; 100 µM CdCl2 for 7 days. Results showed that inoculation with Cd resistant PGPB enhanced plant biomass, thus shoot and root dry weights of control plants were enhanced by 148 and 35% respectively after 7 days. Co-inoculation of plants treated with 50 and 100 µM Cd increased plant biomasses as compared to Cd-treated and uninoculated plants. Cadmium treatment induced lipid peroxidation in plant tissues measured through MDA content in short 7 days 100 µM treatment. Antioxidant enzyme studies showed that inoculation of control plants enhanced APX, SOD and CAT activities after 30 days in shoots and SOD, APX, SOD, GPOX in roots. Application of 50 µM CdCl2 stimulated all enzymes in shoots and decreased SOD and CAT activities in roots. Moreover, 100 µM of CdCl2 increased SOD, APX, CAT and GPOX activities in shoots and increased significantly CAT activity in roots. Metal accumulation depended on Cd concentration, plant organ and time of treatment. Furthermore, the inoculation enhanced Cd uptake in roots by 20% in all treatments. The cultivation of this symbiosis in Cd contaminated soil or in heavy metal hydroponically treated medium, showed that inoculation improved plant biomass and increased Cd uptake especially in roots. Therefore, the present study established that co-inoculation of S. coronaria by a specific consortium of heavy metal resistant PGPB formed a symbiotic system useful for soil phytostabilization.


Cadmium Sulla coronaria Phytostabilization PGPB Symbiosis 



Ascorbate peroxidase




Days after sowing


Dry weight


Ethylene diamine tetra-acetic


Flame atomic absorption spectrophotometer


Fresh weight


Indole acetic acid


Inductively coupled plasma/mass spectrometry




Gluthatione disulfure


Guaiacol peroxidase




Nitroblue tetrazolium


Plant growth promoting bacteria


Plant growth promoting rhizobacteria


Fluorure of phenylmethylsulfonyle




Root dry weight


Reactive oxygen species


Shoot dry weight


Superoxide dismutase


Thiobarbituric acid


Trichloroacetic acid


Unit Forming colony


Yeast extract medium


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Copyright information

© The Botanical Society of Japan and Springer Japan KK 2017

Authors and Affiliations

  • Manel Chiboub
    • 1
  • Salwa Harzalli Jebara
    • 1
  • Omar Saadani
    • 1
  • Imen Challougui Fatnassi
    • 1
  • Souhir Abdelkerim
    • 1
  • Moez Jebara
    • 1
  1. 1.Laboratoire des Légumineuses, Centre de Biotechnologie Borj Cedria University Tunis El ManarHammam LifTunisia

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