Journal of Plant Research

, Volume 130, Issue 3, pp 551–558 | Cite as

Reduced abscisic acid content is responsible for enhanced sucrose accumulation by potassium nutrition in vegetable soybean seeds

  • Bingjie Tu
  • Changkai Liu
  • Bowen Tian
  • Qiuying Zhang
  • Xiaobing Liu
  • Stephen J. Herbert
Regular Paper


In order to understand the physiological mechanism of potassium (K) application in enhancing sugar content of vegetable soybean seeds, pot experiments were conducted in 2014 and 2015 with two vegetable soybean (Glycine max L. Merr.) cultivars (c.v. Zhongkemaodou 1 and c.v. 121) under normal rate of nitrogen and phosphorus application. Three potassium (K) fertilization treatments were imposed: No K application (K0), 120 kg K2SO4 ha−1 at seeding (K1), and 120 kg K2SO4 ha−1 at seedling + 1% K2SO4 foliar application at flowering (K2). Contents of indole-3-acetic acid (IAA), gibberellins (GA), cytokinins (ZR) and abscisic acid (ABA) in seeds were determined from 4 to 8 weeks after flowering. K fertilization increased the contents of IAA, GA, ZR, soluble sugar, sucrose and fresh pod yield, but reduced ABA content consistently. When the contents of soluble sugar and sucrose reached the highest level at 7 weeks after flowering for the 2 cultivars, the contents of IAA、GA、ZR all reached the lowest level in general. The content of ABA in seed was negatively correlated with the sucrose content (P < 0.01, r = −0.749**, −0.768** in 2014 and −0.535**, −0.791** in 2015 for c.v.121 and c.v. Zhongkemaodou 1 respectively). The changes in ratio of the ABA to (IAA + GA + ZR) from 4 to 8 weeks after flowering affected by K application were coincident to the changes of sucrose accumulation. The reduced ratio of ABA/(IAA + GA + ZR) affected by K nutrition particularly reduced abscisic acid content plays a critical role in enhancing sucrose content, which might be a partial mechanism involved in K nutrition to improve the quality of vegetable soybean.


Potassium fertilization Phytohormones Sucrose Vegetable soybean 



This research is partially funded by National Natural Science Foundation of China (Grant No. 41471241) and Major Program of National Science and Technology of China (2016YFD0102105, 2016YFD0100201).


  1. Bauer H, Ache P, Lautner S, Fromm J, Hartung W, Al-Rasheid KA, Mendel RR (2013) The stomatal response to reduced relative humidity requires guard cell-autonomous ABA synthesis. Curr Biol 23:53–57CrossRefPubMedGoogle Scholar
  2. Bhatia S, Singh R (2002) Phytohormone-mediated transformation of sugars to starch in relation to the activities of amylases, sucrose-metabolising enzymes in sorghum grain. Plant Growth Regul 36:97–104CrossRefGoogle Scholar
  3. Cakmak I (2005) The role of potassium in alleviating detrimental effects of abiotic stresses in plants. J Plant Nutr Soil Sci 168:521–530CrossRefGoogle Scholar
  4. Cakmak I, Hengeler C, Marschner H (1994) Changes in phloem export of sucrose in leaves in response to phosphorus, potassium and magnesium deficiency in bean plants. J Exp Bot 45:1251–1257CrossRefGoogle Scholar
  5. Cao Y, Zhao H (2009) Effect of potassium application on forming and accumulation of sucrose in sweet maize. J Northeast Agric Univ 7:008 (Chinese with english summary)Google Scholar
  6. Chen L, Zhu YL, Yang LF, Wei GP, Wang G (2010) Effects of nitrogen forms and ratios on carbon-nitrogen assimilation in developing seeds of vegetable soybean. Acta Bot Boreali-Occident Sin 30:323–329Google Scholar
  7. Cheng Y, Diao F, Wu N, Huang M (1998) Effects of sucrose regulation culture on endogenous ABA levels of carrot somatic embryo. Acta Bot Sin 41:761–765Google Scholar
  8. Davies PJ (2004) Plant hormones: biosynthesis, signal transduction, action!. Springer Science and Business MediaGoogle Scholar
  9. Davies P (2012) Plant hormones and their role in plant growth and development. Springer Science and Business MediaGoogle Scholar
  10. Davies WJ, Jones HG (1991) Abscisic acid physiology and biochemistry. BIOS Scientific PublishersGoogle Scholar
  11. Dong ZX, Fu JM, Zhang YX (2001) Hormone regulating effect for cultivation physiology on soybean. J Shihezi Univ 5:339–341 (Chinese with english summary)Google Scholar
  12. Du M, Li YS, Zhang QY, Gu SY, Liu XB (2012) Effects of potassium fertilizer on chlorophyll content of vegetable soybean in reproductive stages. Soybean Sci 31:941–946Google Scholar
  13. Fehr WR, Caviness CE, Burmood DT, Pennington JS (1971) Stage of development descriptions for soybeans, Glycine max (L.) Merrill. Crop Sci 11:929–931CrossRefGoogle Scholar
  14. Guo HX, Da PZ, Wen SJ (2000) Effects of IAA, GA and ABA on 14 C-sucrose import and metabolism in grape berries. Acta Hortic Sin 27:6–10Google Scholar
  15. Guo Y, Song XL, Wang QC, Li YJ, Sun XZ (2006) Effect of potassium nutrition on endogenous hormone and free radicals in cotton seedling. Acta Agric Boreali-Sinic 1:013Google Scholar
  16. Huber SC, Israel DW (1982) Biochemical basis for partitioning of photosynthetically fixed carbon between starch and sucrose in soybean (glycine-max-merr) leaves. Plant physiol 69:691–696CrossRefPubMedPubMedCentralGoogle Scholar
  17. Iglesias DJ, Tadeo FR, Legaz F, Primo-Millo E, Talon M (2001) In vivo sucrose stimulation of colour change in citrus fruit epicarps: interactions between nutritional and hormonal signals. Physiol Plant 112:244–250Google Scholar
  18. Jaillais Y, Chory J (2010) Unraveling the paradoxes of plant hormone signaling integration. Nat Struct Mol Biol 17:642–645CrossRefPubMedPubMedCentralGoogle Scholar
  19. Ku WZ, Deng KQ, Zhang XQ, Tong JH, Zhou H, Xiao LT (2009) Effects of low potassium stress on mineral nutrient absorption and phytohormone contents of rice seedling. Plant Nutri Ferti Sci 15:69–75Google Scholar
  20. Kurapov PB, Skorobogatova IV, Sal’nikova EI, Sorkina GL, Siusheva AG (1999) Diurnal cycles of endogenous phytohormones in barley. Izvestiia Akademii nauk. Seriia biologicheskaia/Rossiiskaia akademiia nauk 1:108–114Google Scholar
  21. Liu L, Cang J, Yu J, Wang X, Huang R, Wang J, Lu B (2013) Effects of exogenous abscisic acid on carbohydrate metabolism and the expression levels of correlative key enzymes in winter wheat under low temperature. Bios Biotechnol Biochem 77:516–525Google Scholar
  22. Liu CK, Li YS, Tu BJ, Tian BW, Zhang QY, Liu XB (2016) Effects of potassium fertilizer application on soluble sugar content during reproductive stages and yield in vegetable soybean. Soybean Sci 35:270–274Google Scholar
  23. Maathuis FJ (2009) Physiological functions of mineral macronutrients. Curr Opin Plant Biol 12:250–258Google Scholar
  24. Mansfield TA, Jones RJ (1971) Effects of abscisic acid on potassium uptake and starch content of stomatal guard cells. Planta 101:147–158CrossRefPubMedGoogle Scholar
  25. Mohamed AI, Rangappa M (1992) Nutrient composition and anti-nutritional factors in vegetable soybean: II. Oil, fatty acids, sterols, and lipoxygenase activity. Food Chem 44:277–282CrossRefGoogle Scholar
  26. Nemhauser JL, Hong F, Chory J (2006) Different plant hormones regulate similar processes through largely nonoverlapping transcriptional responses. Cell 126:467–475CrossRefPubMedGoogle Scholar
  27. Peuke AD, Jeschke WD, Hartung W (2002) Flows of elements, ions and abscisic acid in Ricinus communis and site of nitrate reduction under potassium limitation. J Exp Bot 53:241–250CrossRefPubMedGoogle Scholar
  28. Qin W, Chen BL, He Q, Si HZ, Li JH (2012) Effects of different ratios of N, P and K fertilizers on endogenous hormone of Xinjiang Fushi apple. J XinJ Agric University 35:373–378 (Chinese with english summary)Google Scholar
  29. Ross JJ, Weston DE, Davidson SE, Reid JB (2011) Plant hormone interactions: how complex are they? Physiol Plant 141:299–309Google Scholar
  30. Santner A, Estelle M (2009) Recent advances and emerging trends in plant hormone signalling. Nature 459:1071–1078CrossRefPubMedGoogle Scholar
  31. Stancheva I, Mitova I, Petkova Z (2004) Effects of different nitrogen fertilizer sources on the yield, nitrate content and other physiological parameters in garden beans. Environ Exp Bot 52:277–282CrossRefGoogle Scholar
  32. Vreugdenhil D (1983) Abscisic acid inhibits phloem loading of sucrose. Physiol Plant 57:463–467Google Scholar
  33. Vyn TJ, Yin X, Bruulsema TW, Jackson CJC, Rajcan I, Brouder SM (2002) Potassium fertilization effects on isoflavone concentrations in soybean [Glycine max (L.) Merr.]. J Agric Food Chem 50:3501–3506CrossRefPubMedGoogle Scholar
  34. Wang YY, Khoo KH, Chen ST, Lin CC, Wong CH, Lin CH (2002) Studies on the immuno-modulating and antitumor activities of Ganoderma lucidum (Reishi) polysaccharides: Functional and proteomic analyses of a fucose-containing glycoprotein fraction responsible for the activities. Bioorgan Med Chem 10:1057–1062CrossRefGoogle Scholar
  35. Wilkinson S, Davies WJ (2002) ABA-based chemical signalling: the co-ordination of responses to stress in plants. Plant Cell Environ 25:195–210CrossRefPubMedGoogle Scholar
  36. Yang J, Zhang J, Wang Z, Zhu Q, Wang W (2001) Hormonal changes in the grains of rice subjected to water stress during grain filling. Plant Physiol 127:315–323CrossRefPubMedPubMedCentralGoogle Scholar
  37. Yinbo G, Peoples MB, Rerkasem B (1997) The effect of N fertilizer strategy on N 2 fixation, growth and yield of vegetable soybean. Field Crop Res 51:221–229CrossRefGoogle Scholar
  38. Zeevaart JAD, Creelman RA (1988) Metabolism and physiology of abscisic acid. Annu Rev Plant Physiol Mol Biol 39:439–473CrossRefGoogle Scholar

Copyright information

© The Botanical Society of Japan and Springer Japan 2017

Authors and Affiliations

  • Bingjie Tu
    • 1
    • 2
  • Changkai Liu
    • 1
  • Bowen Tian
    • 1
    • 2
  • Qiuying Zhang
    • 1
  • Xiaobing Liu
    • 1
    • 2
  • Stephen J. Herbert
    • 3
  1. 1.Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and AgroecologyChinese Academy of SciencesHarbinChina
  2. 2.College of Resources and EnvironmentNortheast Agricultural UniversityHarbinChina
  3. 3.Stockbridge School of AgricultureUniversity of MassachusettsAmherstUSA

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