Journal of Plant Research

, Volume 130, Issue 2, pp 349–363 | Cite as

A subclass of HSP70s regulate development and abiotic stress responses in Arabidopsis thaliana

  • Linna Leng
  • Qianqian Liang
  • Jianjun Jiang
  • Chi Zhang
  • Yuhan Hao
  • Xuelu Wang
  • Wei Su
Regular Paper

Abstract

Members of the HSP70 family function as molecular chaperones to maintain cellular homeostasis and help plants cope with environmental stimuli. However, due to functional redundancy and lack of effective chemical inhibitors, our knowledge of functions of individual HSP70s has remained limited. Here, we confirmed a subclass of HSP70s, including HSP70-1, -2, -3, -4, and -5, localized to the cytosol and nucleus in Arabidopsis thaliana. Histochemical analyses of promoter:GUS reporter lines showed that HSP70-1, -2, -3, and -4 genes were widely expressed, but HSP70-5 was not. In addition, individual HSP70 showed not only similar but also distinct transcriptions when treated by different abiotic stresses and phytohormones. No apparent phenotype was observed when individual HSP70 genes were overexpressed or knocked-out/down, but the double mutant hsp70-1 hsp70-4 and triple mutant hsp70-2 hsp70-4 hsp70-5 plants exhibited developmental phenotypes with shortened specific growth periods, curly and round leaves, twisted petioles, thin stems, and short siliques. Moreover, both mutants were hypersensitive to heat, cold, high glucose, salt and osmotic stress, but hyposensitive to abscisic acid. Genes related to flowering, and the cytokinin, brassinosteroid, and abscisic acid signaling pathways were differentially expressed in both mutants. Our studies suggest that, the individual HSP70 possibly performs both redundant and specific functions with the other members in the cytosolic/nuclear HSP70 subclass, and apart from enabling plants to cope with abiotic stresses, this subclass of cytosolic/nuclear HSP70 proteins also participates in diverse developmental processes and signaling pathways.

Keywords

Abiotic stress Arabidopsis thaliana Development HSP70 Signaling pathway 

Notes

Acknowledgements

We are grateful to the Arabidopsis Biological Resource Center at Ohio State University for providing T-DNA insertion mutants. We are also thankful for help from Mengran Yang and Yangbin Gao in scientific writing. This work was supported by the National Basic Research Program of China (Grant Number 31371231 to W.S.).

Supplementary material

10265_2016_900_MOESM1_ESM.pdf (1.8 mb)
Supplementary material 1 (PDF 1838 KB)

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Copyright information

© The Botanical Society of Japan and Springer Japan 2016

Authors and Affiliations

  • Linna Leng
    • 1
  • Qianqian Liang
    • 1
  • Jianjun Jiang
    • 1
  • Chi Zhang
    • 1
  • Yuhan Hao
    • 1
  • Xuelu Wang
    • 2
  • Wei Su
    • 1
  1. 1.State Key Laboratory of Genetic Engineering and Institute of Plant Biology, School of Life SciencesFudan UniversityShanghaiChina
  2. 2.College of Life Science and TechnologyHuazhong Agricultural UniversityWuhanChina

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