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Journal of Plant Research

, Volume 130, Issue 2, pp 397–405 | Cite as

Plasma membrane-anchored chloroplasts are necessary for the gravisensing system of Ceratopteris richardii prothalli

  • Hiroyuki Kamachi
  • Daisuke Tamaoki
  • Ichirou Karahara
Regular Paper
  • 390 Downloads

Abstract

The prothalli of the fern Ceratopteris richardii exhibit negative gravitropism when grown in darkness. However, no sedimentable organelles or substances have been detected in the prothallial cells, suggesting that a non-sedimentable gravisensor exists. We investigated whether chloroplasts are involved in the gravisensing system of C. richardii prothalli. We used a clumped-chloroplast mutant, clumped chloroplast 1 (cp1), in which the chloroplasts are detached from the plasma membrane and clustered around the nucleus likely because of a partial deletion in the KINESIN-LIKE PROTEIN FOR ACTIN-BASED CHLOROPLAST MOVEMENT 1 gene. The cp1 mutation resulted in prothalli that had a significantly diminished gravitropic response, while the phototropic response occurred normally. These results suggest that plasma membrane-anchored chloroplasts in prothallial cells function as one of the gravisensors in C. richardii prothalli.

Keywords

Ceratopteris richardii Fern gametophyte Gravitropism Gravity perception 

Notes

Acknowledgements

This work was supported by JSPS KAKENHI Grant Number JP15K11914.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10265_2016_889_MOESM1_ESM.pdf (227 kb)
Supplementary material 1 (PDF 227 KB)

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Copyright information

© The Botanical Society of Japan and Springer Japan 2016

Authors and Affiliations

  • Hiroyuki Kamachi
    • 1
  • Daisuke Tamaoki
    • 1
    • 2
  • Ichirou Karahara
    • 1
  1. 1.Graduate School of Science and EngineeringUniversity of ToyamaToyamaJapan
  2. 2.Division of Functional Genomics, Advanced Science Research CenterKanazawa UniversityKanazawaJapan

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