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Journal of Plant Research

, Volume 129, Issue 5, pp 1005–1010 | Cite as

Identifying sex in non-fertile individuals of the moss Drepanocladus turgescens (Bryophyta: Amblystegiaceae) using a novel molecular approach

  • Lars Hedenäs
  • Helena Korpelainen
  • Irene Bisang
Technical Note

Abstract

Sex identification before sexual maturity is notoriously difficult in plants with separate sexes, but is crucial to address many life history related issues. To study the performance of the two sexes in the rarely sexually reproducing dioecious moss Drepanocladus turgescens a molecular sex marker is needed. The female-targeting marker previously developed for D. trifarius and D. lycopodioides amplifies for a few D. turgescens males, which can thus not be distinguished from females. In a significant addition to the earlier developed method we sequenced the portion successfully amplified by the primers PT−3f and PT−3r for six females and three males. Differences between males and females were revealed at five sequence positions. Examination of a total of fourteen females and seven marker amplifying males confirm that females and such males differ consistently at these positions. The usefulness of a previous protocol for moss sex identification is thus extended to another dioecious moss by the addition of a step where a portion of the sex-correlated region is sequenced.

Keywords

Biased sex ratio Genetic sex ratio Molecular sex marker New sequencing step 

Notes

Acknowledgments

We thank K. Mirbakhsh and M. Pietiläinen for their efficient labwork. Two reviewers provided valuable comments to an earlier version of the manuscript.

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Copyright information

© The Botanical Society of Japan and Springer Japan 2016

Authors and Affiliations

  • Lars Hedenäs
    • 1
  • Helena Korpelainen
    • 2
  • Irene Bisang
    • 1
  1. 1.Department of BotanySwedish Museum of Natural HistoryStockholmSweden
  2. 2.Department of Agricultural SciencesUniversity of HelsinkiHelsinkiFinland

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