Journal of Plant Research

, Volume 129, Issue 4, pp 711–726 | Cite as

Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray

  • Yoshinori Utsumi
  • Maho Tanaka
  • Atsushi Kurotani
  • Takuhiro Yoshida
  • Keiichi Mochida
  • Akihiro Matsui
  • Manabu Ishitani
  • Supajit Sraphet
  • Sukhuman Whankaew
  • Thipa Asvarak
  • Jarunya Narangajavana
  • Kanokporn Triwitayakorn
  • Tetsuya Sakurai
  • Motoaki Seki
Regular Paper

Abstract

Cassava anthracnose disease (CAD), caused by the fungus Colletotrichum gloeosporioides f. sp. Manihotis, is a serious disease of cassava (Manihot esculenta) worldwide. In this study, we established a cassava oligonucleotide-DNA microarray representing 59,079 probes corresponding to approximately 30,000 genes based on original expressed sequence tags and RNA-seq information from cassava, and applied it to investigate the molecular mechanisms of resistance to fungal infection using two cassava cultivars, Huay Bong 60 (HB60, resistant to CAD) and Hanatee (HN, sensitive to CAD). Based on quantitative real-time reverse transcription PCR and expression profiling by the microarray, we showed that the expressions of various plant defense-related genes, such as pathogenesis-related (PR) genes, cell wall-related genes, detoxification enzyme, genes related to the response to bacterium, mitogen-activated protein kinase (MAPK), genes related to salicylic acid, jasmonic acid and ethylene pathways were higher in HB60 compared with HN. Our results indicated that the induction of PR genes in HB60 by fungal infection and the higher expressions of defense response-related genes in HB60 compared with HN are likely responsible for the fungal resistance in HB60. We also showed that the use of our cassava oligo microarray could improve our understanding of cassava molecular mechanisms related to environmental responses and development, and advance the molecular breeding of useful cassava plants.

Keywords

Cassava Cassava anthracnose disease Detoxification Disease resistance Transcriptome 

Notes

Acknowledgments

This work was supported by the Japan Science and Technology Agency (JST) and the National Science and Technology Development Agency of Thailand (Grant No.P12-01382) under the East Asia Science and Innovation Area Joint Research Program (e-ASIA JRP). This research was also supported by the Strategic Funds for the Promotion of Science and Technology of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan; RIKEN Center for Sustainable Resource Science (CSRS); and CREST, JST, Japan. JN and KT were supported by the National Science and Technology Development Agency, Thailand, (Grant No. P-12-01382), under the East Asia Science and Innovation Area Joint Research Program (e-ASIA JRP).

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Copyright information

© The Botanical Society of Japan and Springer Japan 2016

Authors and Affiliations

  • Yoshinori Utsumi
    • 1
  • Maho Tanaka
    • 1
  • Atsushi Kurotani
    • 2
  • Takuhiro Yoshida
    • 2
  • Keiichi Mochida
    • 3
    • 4
  • Akihiro Matsui
    • 1
  • Manabu Ishitani
    • 5
  • Supajit Sraphet
    • 6
  • Sukhuman Whankaew
    • 6
  • Thipa Asvarak
    • 7
  • Jarunya Narangajavana
    • 7
  • Kanokporn Triwitayakorn
    • 6
  • Tetsuya Sakurai
    • 2
    • 8
  • Motoaki Seki
    • 1
    • 9
    • 10
  1. 1.Plant Genomic Network Research TeamRIKEN Center for Sustainable Resource ScienceYokohamaJapan
  2. 2.Integrated Genome Informatics Research UnitRIKEN Center for Sustainable Resource ScienceYokohamaJapan
  3. 3.Gene Discovery Research GroupRIKEN Center for Sustainable Resource ScienceYokohamaJapan
  4. 4.Biomass Research Platform TeamRIKEN Biomass Engineering ProgramYokohamaJapan
  5. 5.Agrobiodiversity Research AreaInternational Center for Tropical Agriculture (CIAT)CaliColombia
  6. 6.Institute of Molecular BiosciencesMahidol UniversitySalayaThailand
  7. 7.Department of Biotechnology, Faculty of ScienceMahidol UniversityBangkokThailand
  8. 8.Research and Education Faculty, Multidisciplinary Science ClusterInterdisciplinary Science UnitNankokuJapan
  9. 9.Core Research for Evolutional Science and Technology (CREST)Japan Science and Technology (JST)KawaguchiJapan
  10. 10.Kihara Institute for Biological ResearchYokohama City UniversityYokohamaJapan

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