Ichthyological Research

, Volume 65, Issue 2, pp 276–281 | Cite as

Rapid detection of alien DNA in the loach genus Misgurnus (family Cobitidae) using real-time PCR

  • Takaaki Shimizu
  • Kimiyoshi Narita
  • Norio Onikura
News and Comments

Cryptic alien introduction of non-native species and genetic material into native populations can disrupt assessment of species diversity and population structure, and implementation of conservation programs. Interspecific alien introduction among morphologically indistinct congeners often occurs cryptically, and leads to irreversible ecological disturbances (Konishi 2010). Intraspecific immigration among local populations also complicates the natural history of resident populations (Takehana 2010). In Japan, exotic seed of the loach genus Misgurnus (family Cobitidae) was imported from China for the purposes of aquaculture and as fishing bait (Saito et al. 2011; Shimizu 2014). In addition, Misgurnus anguillicaudatus with alien DNA, and alien species Misgurnus dabryanus –scientific name of the latter species tentatively follows Hosoya (2013) due to its historically confusing - have been observed widely in Japanese rice paddies, irrigation channels, streams and ponds (Kanou et al. 2007;...



We thank Y. Suzawa for providing us some Misgurnus specimens. This work was supported by JSPS KAKENHI Grant Number JP 26250044.

Supplementary material

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Supplementary material 1 (DOCX 15 kb)
10228_2017_609_MOESM2_ESM.docx (16 kb)
Supplementary material 2 (DOCX 16 kb)
10228_2017_609_MOESM3_ESM.docx (18 kb)
Supplementary material 3 (DOCX 18 kb)


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Copyright information

© The Ichthyological Society of Japan 2017

Authors and Affiliations

  • Takaaki Shimizu
    • 1
  • Kimiyoshi Narita
    • 1
  • Norio Onikura
    • 2
  1. 1.Ehime Prefectural Institute of FisheriesIyoJapan
  2. 2.Fishery Research LaboratoryKyushu UniversityFukutsuJapan

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