Nitrification-related factors involved in the biochemical oxygen demand of leachate from inert-waste landfill site

  • Shusaku HirakawaEmail author
  • Tomoko Koga
  • Nobuhiro Shimizu
  • Tsuguhide Hori
  • Youichi Kurokawa
  • Mineki Toba


The 5-day biochemical oxygen demand (BOD5) is an organic matter indicator used for the maintenance management of inert-waste landfill sites. Previously, we indicated that oxygen consumption derived from the nitrification reaction (N-BOD5) might have a non-negligible contribution to BOD5 in leachate. In this study, we investigated changes over time of organic matter indicators in leachate of an inert-waste landfill site, and searched for factors responsible for fluctuations of N-BOD5. Water quality was measured in two kinds of leachate, before (LBA) and after aeration (LAA), each month from September 2011 to February 2016. The N-BOD5 component of BOD5 in LAA was higher than that of LBA. The N-BOD5 in LAA showed a significant positive correlation with ammonium nitrogen (NH4-N), and NH4-N in LAA also had a significant positive correlation with precipitation. As a result of NH4-N addition test, N-BOD5 in LAA was increased, but N-BOD5 in LBA was hardly affected. Furthermore, the composition ratio of nitrifying bacteria in LAA was higher than that in LBA. Therefore, it was suggested that fluctuations of N-BOD5 are related to the aerobic condition of the environment, NH4-N levels and the presence of nitrifying bacteria, and are indirectly influenced by precipitation.


Leachate Biochemical oxygen demand Nitrification Ammonium nitrogen Microbial community 



This work was supported by JSPS KAKENHI Grant number JP16K21716.

Supplementary material

10163_2019_885_MOESM1_ESM.pdf (87 kb)
Supplementary material 1 (PDF 87 kb)
10163_2019_885_MOESM2_ESM.pdf (79 kb)
Supplementary material 2 (PDF 79 kb)


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© Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Fukuoka Institute of Health and Environmental SciencesDazaifuJapan

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