Thermophilic anaerobic digestion is an effective treatment for reducing cefazolin-resistant bacteria and ESBL-producers in dairy manure
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The application of thermophilic anaerobic digestion (TAD) treatments to dairy manure in a biogas plant was evaluated to investigate whether the prominent countermeasure was sufficient for the dissemination of antimicrobial-resistant (AMR) bacteria in the dairy industry. To determine the changes in the number of AMR bacteria in dairy manure after TAD, cefazolin-resistant (CEZ-R) and ampicillin-resistant (AMP-R) bacteria in dairy manure and digestate were quantified by plate spread methods performed with combinations of antimicrobials added to agar plates and selective and differential agar plates. In addition, extended-spectrum β-lactamase (ESBL)-producing bacteria were also quantified to evaluate the effect of TAD on plasmid-borne resistant bacteria. CEZ-R and AMP-R bacteria were widely reduced after TAD compared with the susceptible bacteria against these antimicrobials. The classification into E. coli, other coliforms, and non-coliform bacteria revealed that CEZ-R and AMP-R coliform bacteria were more significantly reduced than non-coliform bacteria by TAD. Moreover, ESBL-producing bacteria were reduced significantly among CEZ-R bacteria. From these results, TAD appears to be a useful treatment to counter the dissemination of AMR bacteria in dairy manure by the preferential elimination of AMR bacteria.
KeywordsAnaerobic digestion Antimicrobial-resistant bacteria Cefazolin Digestate Extended-spectrum β-lactamase
This study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (No. 10670499).
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