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Thermophilic anaerobic digestion is an effective treatment for reducing cefazolin-resistant bacteria and ESBL-producers in dairy manure

  • Masahiro Iwasaki
  • Masazumi Miyake
  • Hideaki Maseda
  • Guangdou Qi
  • Zhifei Pan
  • Ikko Ihara
  • Kazutaka UmetsuEmail author
ORIGINAL ARTICLE
  • 108 Downloads

Abstract

The application of thermophilic anaerobic digestion (TAD) treatments to dairy manure in a biogas plant was evaluated to investigate whether the prominent countermeasure was sufficient for the dissemination of antimicrobial-resistant (AMR) bacteria in the dairy industry. To determine the changes in the number of AMR bacteria in dairy manure after TAD, cefazolin-resistant (CEZ-R) and ampicillin-resistant (AMP-R) bacteria in dairy manure and digestate were quantified by plate spread methods performed with combinations of antimicrobials added to agar plates and selective and differential agar plates. In addition, extended-spectrum β-lactamase (ESBL)-producing bacteria were also quantified to evaluate the effect of TAD on plasmid-borne resistant bacteria. CEZ-R and AMP-R bacteria were widely reduced after TAD compared with the susceptible bacteria against these antimicrobials. The classification into E. coli, other coliforms, and non-coliform bacteria revealed that CEZ-R and AMP-R coliform bacteria were more significantly reduced than non-coliform bacteria by TAD. Moreover, ESBL-producing bacteria were reduced significantly among CEZ-R bacteria. From these results, TAD appears to be a useful treatment to counter the dissemination of AMR bacteria in dairy manure by the preferential elimination of AMR bacteria.

Keywords

Anaerobic digestion Antimicrobial-resistant bacteria Cefazolin Digestate Extended-spectrum β-lactamase 

Notes

Acknowledgements

This study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (No. 10670499).

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Copyright information

© Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • Masahiro Iwasaki
    • 1
  • Masazumi Miyake
    • 2
  • Hideaki Maseda
    • 3
  • Guangdou Qi
    • 1
  • Zhifei Pan
    • 2
  • Ikko Ihara
    • 4
  • Kazutaka Umetsu
    • 1
    Email author
  1. 1.Department of Agro-Environmental ScienceObihiro University of Agriculture and Veterinary MedicineObihiroJapan
  2. 2.Department of Animal and Food HygieneObihiro University of Agriculture and Veterinary MedicineObihiroJapan
  3. 3.Department of Bioresource Chemistry and TechnologyTokushima UniversityTokushimaJapan
  4. 4.Department of Agricultural Engineering and Socio-EconomicsKobe UniversityKobeJapan

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