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Thermophilic anaerobic digestion is an effective treatment for reducing cefazolin-resistant bacteria and ESBL-producers in dairy manure

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Abstract

The application of thermophilic anaerobic digestion (TAD) treatments to dairy manure in a biogas plant was evaluated to investigate whether the prominent countermeasure was sufficient for the dissemination of antimicrobial-resistant (AMR) bacteria in the dairy industry. To determine the changes in the number of AMR bacteria in dairy manure after TAD, cefazolin-resistant (CEZ-R) and ampicillin-resistant (AMP-R) bacteria in dairy manure and digestate were quantified by plate spread methods performed with combinations of antimicrobials added to agar plates and selective and differential agar plates. In addition, extended-spectrum β-lactamase (ESBL)-producing bacteria were also quantified to evaluate the effect of TAD on plasmid-borne resistant bacteria. CEZ-R and AMP-R bacteria were widely reduced after TAD compared with the susceptible bacteria against these antimicrobials. The classification into E. coli, other coliforms, and non-coliform bacteria revealed that CEZ-R and AMP-R coliform bacteria were more significantly reduced than non-coliform bacteria by TAD. Moreover, ESBL-producing bacteria were reduced significantly among CEZ-R bacteria. From these results, TAD appears to be a useful treatment to counter the dissemination of AMR bacteria in dairy manure by the preferential elimination of AMR bacteria.

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Acknowledgements

This study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (No. 10670499).

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Authors and Affiliations

  1. Department of Agro-Environmental Science, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada-cho, Obihiro, Hokkaido, 080-8555, Japan

    Masahiro Iwasaki, Guangdou Qi & Kazutaka Umetsu

  2. Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan

    Masazumi Miyake & Zhifei Pan

  3. Department of Bioresource Chemistry and Technology, Tokushima University, Tokushima, Japan

    Hideaki Maseda

  4. Department of Agricultural Engineering and Socio-Economics, Kobe University, Kobe, Japan

    Ikko Ihara

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  1. Masahiro Iwasaki
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  2. Masazumi Miyake
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  3. Hideaki Maseda
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  4. Guangdou Qi
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  5. Zhifei Pan
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  6. Ikko Ihara
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  7. Kazutaka Umetsu
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Correspondence to Kazutaka Umetsu.

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Iwasaki, M., Miyake, M., Maseda, H. et al. Thermophilic anaerobic digestion is an effective treatment for reducing cefazolin-resistant bacteria and ESBL-producers in dairy manure. J Mater Cycles Waste Manag 21, 293–299 (2019). https://doi.org/10.1007/s10163-018-0789-3

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  • DOI: https://doi.org/10.1007/s10163-018-0789-3

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