Evaluation of the physiology of miniature pig fed Shochu distillery waste using mRNA expression profiling
Shochu distillery waste is the discarded material generated during Shochu production, and it has been used as feed for livestock. In this study, we evaluated the applicability of Shochu distillery waste as a livestock diet. Shochu distillery waste diet was fed to Clawn miniature pigs. At the end of the dietary period, blood samples were collected for biochemical examination and microarray analysis. No significant differences were observed between the control and Shochu distillery waste treatment groups based on physical and biochemical examination. Gene expression patterns were also similar. In addition, gene profiling of these two groups was compared with those of hyperlipidemia and toxicant model groups. Expression profiling of the two groups was different from those of the hyperlipidemia and toxicant model groups. In conclusion, the Shochu distillery waste diet did not affect pig physiology and it is a suitable substitute for standard feed. Moreover, these results promote the potential for microarray analysis use in the evaluation of food safety.
KeywordsBlood DNA microarray Gene expression Pig Shochu
The authors wish to thank Okuchi Liquor Co., Ltd. for supplying the SDW, and Junichi Tottori and Takehiro Iwanaga with Japan Farm Co., Ltd. for breeding the animals and collecting the data.
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