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Interaction of H. pylori with toll-like receptor 2-196 to -174 ins/del polymorphism is associated with gastric cancer susceptibility in southern China

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Abstract

Background

Genetic polymorphisms of Toll-like receptors play important roles in gastric carcinogenesis. The aim of this study was to determine the role of TLR2-196 to -174 ins/del polymorphism in gastric cancer susceptibility and prognosis.

Methods

This study included 520 people from southern China. Samples were genotyped by the allele-specific polymerase chain reaction, among which 10% were randomly selected for sequencing. The serological method was used to determine Helicobacter pylori.

Results

The TLR2 genotype was not associated with the risk of H. pylori infection. The del/del genotype exhibited significantly higher gastric cancer risk (adjusted OR 2.59, 95% CI 1.33‒5.07) than that of the ins/ins genotype. Further stratification analyses demonstrated that the del/del genotype was associated with a risk of intestinal gastric cancer (adjusted OR 2.62, 95% CI 1.34–5.14). In addition, the presence of the del/del genotype and the H. pylori infection conferred a synergistic effect (OR 3.04, 95% CI 1.33‒6.98) for the development of gastric cancer. The del/del genotype was not associated with a poor prognosis in gastric cancer patients.

Conclusion

The del/del genotype is associated with an increased gastric cancer risk in the southern Chinese population. However, TLR2 polymorphism is neither associated with H. pylori infection, nor with a poor prognosis.

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Acknowledgements

This study was supported by the Applied Basic Research Fund of Changzhou, China (CJ20140022, CJ20179034), Young medical talents of Jiangsu province (QNRC2016269), the High-level Medicine Talents Training Project (2016CZBJ022), and the Youth Science and Technology Project of Changzhou Health and Family Planning Commission (QN201601).

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Author notes

  1. Jin-Huang and Jun-Jie Hang contributed equally to this study.

Authors and Affiliations

  1. Department of Gastroenterology, Changzhou No. 2 People’s Hospital, Xinglong Xiang 29, Changzhou, 213000, Jiangsu, China

    Jin Huang, Xiang-Rong Qin, Jian Huang & Xiao-Yong Wang

  2. Department of Oncology, Changzhou No. 2 People’s Hospital, Xinglong Xiang 29, Changzhou, 213000, China

    Jun-Jie Hang

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  1. Jin Huang
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  2. Jun-Jie Hang
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  3. Xiang-Rong Qin
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  4. Jian Huang
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  5. Xiao-Yong Wang
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Correspondence to Xiao-Yong Wang.

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Huang, J., Hang, JJ., Qin, XR. et al. Interaction of H. pylori with toll-like receptor 2-196 to -174 ins/del polymorphism is associated with gastric cancer susceptibility in southern China. Int J Clin Oncol 24, 494–500 (2019). https://doi.org/10.1007/s10147-018-1379-z

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  • DOI: https://doi.org/10.1007/s10147-018-1379-z

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