Identification of a Sorbicillinoid-Producing Aspergillus Strain with Antimicrobial Activity Against Staphylococcus aureus: a New Polyextremophilic Marine Fungus from Barents Sea
The exploration of poorly studied areas of Earth can highly increase the possibility to discover novel bioactive compounds. In this study, the cultivable fraction of fungi and bacteria from Barents Sea sediments has been studied to mine new bioactive molecules with antibacterial activity against a panel of human pathogens. We isolated diverse strains of psychrophilic and halophilic bacteria and fungi from a collection of nine samples from sea sediment. Following a full bioassay-guided approach, we isolated a new promising polyextremophilic marine fungus strain 8Na, identified as Aspergillus protuberus MUT 3638, possessing the potential to produce antimicrobial agents. This fungus, isolated from cold seawater, was able to grow in a wide range of salinity, pH and temperatures. The growth conditions were optimised and scaled to fermentation, and its produced extract was subjected to chemical analysis. The active component was identified as bisvertinolone, a member of sorbicillonoid family that was found to display significant activity against Staphylococcus aureus with a minimum inhibitory concentration (MIC) of 30 μg/mL.
KeywordsSediments Marine fungi Aspergillus protuberus Bisvertinolone Antimicrobial activity MDR
P.C., F.P.E. and P.T. equally contributed to the work. P.C., F.P.E, A.F. and D.d.P. conceived and designed the experiments; P.C., F.P.E., P.T., A.F., E.T., L.T., M.V.D.A., G.G. and G.C.V. performed the experiments; P.C., F.P.E, P.T., A.F. and D.d.P. analysed the data; G.C.V., L.T., M.V.D.A. and D.d.P. contributed reagent/material/analysis tools; P.C., F.P.E., P.T. and D.d.P. wrote the paper.
This work was supported by the EU FP7 KBBE2012-2016 project: “Pharmasea,” Grant Agreement 312184, and by the H2020-MSCA-RISE: Ocean Medicines, Grant Agreement 690944.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
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