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Marine Biotechnology

, Volume 20, Issue 4, pp 502–511 | Cite as

Identification of a Sorbicillinoid-Producing Aspergillus Strain with Antimicrobial Activity Against Staphylococcus aureus: a New Polyextremophilic Marine Fungus from Barents Sea

  • Paulina Corral
  • Fortunato Palma Esposito
  • Pietro Tedesco
  • Angela Falco
  • Emiliana Tortorella
  • Luciana Tartaglione
  • Carmen Festa
  • Maria Valeria D’Auria
  • Giorgio Gnavi
  • Giovanna Cristina Varese
  • Donatella de Pascale
Original Article

Abstract

The exploration of poorly studied areas of Earth can highly increase the possibility to discover novel bioactive compounds. In this study, the cultivable fraction of fungi and bacteria from Barents Sea sediments has been studied to mine new bioactive molecules with antibacterial activity against a panel of human pathogens. We isolated diverse strains of psychrophilic and halophilic bacteria and fungi from a collection of nine samples from sea sediment. Following a full bioassay-guided approach, we isolated a new promising polyextremophilic marine fungus strain 8Na, identified as Aspergillus protuberus MUT 3638, possessing the potential to produce antimicrobial agents. This fungus, isolated from cold seawater, was able to grow in a wide range of salinity, pH and temperatures. The growth conditions were optimised and scaled to fermentation, and its produced extract was subjected to chemical analysis. The active component was identified as bisvertinolone, a member of sorbicillonoid family that was found to display significant activity against Staphylococcus aureus with a minimum inhibitory concentration (MIC) of 30 μg/mL.

Keywords

Sediments Marine fungi Aspergillus protuberus Bisvertinolone Antimicrobial activity MDR 

Notes

Author Contributions

P.C., F.P.E. and P.T. equally contributed to the work. P.C., F.P.E, A.F. and D.d.P. conceived and designed the experiments; P.C., F.P.E., P.T., A.F., E.T., L.T., M.V.D.A., G.G. and G.C.V. performed the experiments; P.C., F.P.E, P.T., A.F. and D.d.P. analysed the data; G.C.V., L.T., M.V.D.A. and D.d.P. contributed reagent/material/analysis tools; P.C., F.P.E., P.T. and D.d.P. wrote the paper.

Funding Information

This work was supported by the EU FP7 KBBE2012-2016 project: “Pharmasea,” Grant Agreement 312184, and by the H2020-MSCA-RISE: Ocean Medicines, Grant Agreement 690944.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

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Figure S1 (PDF 198 kb)
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Figure S3 (PDF 52 kb)
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Figure S5 (PDF 50 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Paulina Corral
    • 1
    • 2
  • Fortunato Palma Esposito
    • 1
  • Pietro Tedesco
    • 1
  • Angela Falco
    • 1
  • Emiliana Tortorella
    • 1
  • Luciana Tartaglione
    • 3
  • Carmen Festa
    • 3
  • Maria Valeria D’Auria
    • 3
  • Giorgio Gnavi
    • 4
  • Giovanna Cristina Varese
    • 4
  • Donatella de Pascale
    • 1
  1. 1.National Research Council of Italy (CNR)-Institute of Protein Biochemistry (IBP)NaplesItaly
  2. 2.Burnett School of Biomedical Sciences, College of MedicineUniversity of Central FloridaOrlandoUSA
  3. 3.Department of PharmacyUniversity of Naples Federico IINaplesItaly
  4. 4.Department of Life Science and Systems BiologyUniversity of TorinoTurinItaly

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