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Degradative properties of two newly isolated strains of the ascomycetes Fusarium oxysporum and Lecanicillium aphanocladii

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Abstract

Two ascomycete strains were isolated from creosote-contaminated railway sleeper wood. By using a polyphasic approach combining morpho-physiological observations of colonies with molecular tools, the strains were identified as Fusarium oxysporum Schltdl. (IBPPM 543, MUT 4558; GenBank accession no. MG593980) and Lecanicillium aphanocladii Zare & W. Gams (IBPPM 542, MUT 242; GenBank accession no. MG593981). Both strains degraded hazardous pollutants, including polycyclic aromatic hydrocarbons, anthraquinone-type dyes, and oil. Oil was better degraded by F. oxysporum, but the aromatic compounds were better degraded by L. aphanocladii. With both strains, the degradation products of anthracene, phenanthrene, and fluorene were 9,10-anthraquinone, 9,10-phenanthrenequinone, and 9-fluorenone, respectively. During pollutant degradation, F. oxysporum and L. aphanocladii produced an emulsifying compound(s). Both fungi produced extracellular Mn-peroxidases, enzymes possibly involved in the fungal degradation of the pollutants. This is the first report on the ability of L. aphanocladii to degrade four-ring PAHs, anthraquinone-type dyes, and oil, with the simultaneous production of an extracellular Mn-peroxidase.

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Acknowledgements

We are grateful to Dr. M.P. Chernyshova (IBPPM RAS) for gas chromatographic analysis. We are also grateful to Dmitry N. Tychinin for his assistance in preparation of the English text of this paper.

Funding

This research was supported by a grant from the Russian Science Foundation (project no. 16-14-00081).

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov, Russia, 410049

    Natalia N. Pozdnyakova, Ekaterina V. Dubrovskaya, Svetlana A. Balandina & Olga V. Turkovskaya

  2. Department of Life Sciences and Systems Biology, University of Turin, Turin, Italy

    Giovanna C. Varese & Valeria Prigione

Authors
  1. Natalia N. Pozdnyakova
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  2. Giovanna C. Varese
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  3. Valeria Prigione
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  4. Ekaterina V. Dubrovskaya
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  5. Svetlana A. Balandina
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  6. Olga V. Turkovskaya
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Correspondence to Natalia N. Pozdnyakova.

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Pozdnyakova, N.N., Varese, G.C., Prigione, V. et al. Degradative properties of two newly isolated strains of the ascomycetes Fusarium oxysporum and Lecanicillium aphanocladii. Int Microbiol 22, 103–110 (2019). https://doi.org/10.1007/s10123-018-0032-z

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  • DOI: https://doi.org/10.1007/s10123-018-0032-z

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