In women, serum levels of CTSB, GKN2, LIPF, LIPFG, AZGP1, TOP2A and PGA4 are proposed as predictive markers of gastric cancer. It is unknown whether GKN1 expression varies with the sex of patients with chronic gastritis or gastric cancer. We studied 36 patients with histopathological diagnosis of chronic gastritis from the state of Guerrero, Mexico. PCR was performed for H. pylori detection and GKN1 expression was determined by RT-qPCR and western blot. GKN1 mRNA expression was significantly lower in patients with chronic follicular gastritis than in those with chronic chemical gastritis (p = 0.00071). The mRNA and protein level of expression of GKN1 were significantly lower in women with chronic follicular gastritis than in men with the same condition (p = 0.0279 and p = 0.0014, respectively); the lowest levels of GKN1 were detected in women with H. pylori-positive follicular gastritis (p = 0.0175 and p = 0.0111, respectively). Through a bioinformatic analysis, estrogen response elements were identified in the GKN1 promoter.
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We express our sincere gratitude to all patients for their participation in the study, as well as to the nurses and staff of the Instituto Estatal de Cancerología who supported obtention of the biopsies. We thank. Josefina Atrisco Morales, MSc. and Ilce Valeria Román Fernández, PhD. for the support they provided in conducting this investigation.
The research was carried out with the funding granted by CONACYT to Project no. 258433, in the call for Basic Scientific Research 2015–01 and with the resources granted by the Program for Strengthening Educational Quality (PFCE-SEP-2017 and 2018), of the Ministry of Public Education, to the consolidated academic body Infectious Agents and Cancer (UAGro-CA-194). Judit Alarcón-Millán is a PhD student in biomedical sciences and she is receiving a doctoral fellowship from CONACYT, Mexico (Grant no. 296364). Sandra Ines Lorenzo-Nazario was a fellow of CONACYT while doing her masters in biomedical sciences (Grant no. 777741).
Conflict of interest
The authors have no conflict of interest.
This study was approved by the Bioethics Committee of the Universidad Autónoma de Guerrero and by the Research Department of the Instituto Estatal de Cancerología.
Informed consent was obtained from all the participants.
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Alarcón-Millán, J., Lorenzo-Nazario, S.I., Jiménez-Wences, H. et al. Women with chronic follicular gastritis positive for Helicobacter pylori express lower levels of GKN1. Gastric Cancer 23, 754–759 (2020). https://doi.org/10.1007/s10120-020-01049-5
- Gastrokine 1
- Chronic gastritis
- Helicobacter pylori