Investigation of LED-based photodynamic therapy efficiency on breast cancer cells


Photodynamic therapy (PDT) is based on special light source, photosensitizer (PS), and in the presence of oxygen. Different light sources have been used for PDT applications. Recent studies have focused on LED light sources for PDT applications due to reducing the cost of laser-based PDT and providing easy access for research laboratory or clinic facilities. LED-mediated PDT applications have shown promising results for the treatment of different types of disease. However, few studies have determined the effects of LED-based PDT on cancer cells. For the first time, the aim of this study was to explore the therapeutic effects of 5-aminolevulinic acid (5-ALA)-mediated PDT after LED irradiation on two sub-types (a poorly aggressive MCF-7 and a highly aggressive MDA-MB-231) of breast cancer cell lines. The effectiveness of 5-ALA PDT treatment was evaluated by WST-1, annexin V, and acridine orange staining with different energy levels. The LED system was specially developed with optical power and wavelength stability techniques. The system consists of user interface and embedded LED controller with real-time optic power output calibration by photodiode feedback. Our results demonstrated that the cell viability of breast cancer cells was considerably decreased a LED dose-dependent manner (P < 0.05). Additionally, a significant increase in the percentage of apoptotic cells was detected in breast cancer cells after irradiation with LED at a density of 18 and 30 J/cm2 energy. Consequently, the LED system could be effectively used for irradiation of 5-ALA in the treatment of breast cancer cells.

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This work was supported by the Scientific and Technological Research Council of Turkey, TUBITAK 3501 (no. 118E235).

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All authors contributed to the study conception and design. Experimental analysis were performed by Ali Furkan Kamanlı, Ebru Özyol, Asuman Deveci Ozkan, Elif Sözen Kucukkara, and Gamze Guney Eskiler. The data analysis were performed by Mustafa Zahid Yıldız. The first draft of the manuscript was written by Ali Furkan Kamanlı and Gamze Guney Eskiler, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Gamze Guney Eskiler.

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Kamanlı, A.F., Yıldız, M.Z., Özyol, E. et al. Investigation of LED-based photodynamic therapy efficiency on breast cancer cells. Lasers Med Sci (2020).

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  • Photodynamic therapy
  • 5-aminolevulinic acid
  • Light-emitting diodes (LEDs)
  • Breast cancer